银杏叶提取物对人胚肺成纤维细胞氧化应激的保护作用  

Protective effect of ginkgo biloba extract on human embryo pulmonary fibroblasts under chronic mild oxidative stress in vitro

作　　者：潘尚领[1] 刘承武[1] 黄龄瑾[1] 张锡流[2] 陈晶[1] 罗晓秋[1] 李辉[1] 甘瑞静[1] 陈文成[1] 

机构地区：[1]广西医科大学病理生理学教研室,广西南宁530021 [2]广西中医学院第一附属医院病理科

出　　处：《中国老年学杂志》2007年第12期1128-1130,共3页Chinese Journal of Gerontology

基　　金：广西教育厅项目(桂教科研〔2003〕22号);广西科技厅项目(桂科青0542044;0447029)

摘　　要：目的探讨银杏提取物(EGb)对体外培养的人类成纤维细胞的抗氧化作用。方法分别于常态氧(氧浓度为21%)及高氧(氧浓度为40%)环境下培养人胚肺成纤维细胞(MRC-5),并给予不同浓度(0,7.5,15,30,60μg/ml)的EGb,以400μmol/L的α-苯基-N-4-丁基硝酮(PBN)为阳性对照,观察不同条件下细胞的生长曲线,并用流式细胞仪测定不同时期、不同条件下MRC-5细胞内活性氧(ROS)的含量。结果常规培养条件下,MRC-5在不同浓度EGb下的生长曲线无明显差别,不同时期测定的ROS含量与阴性或阳性对照比较,总体上无显著差异;而在高氧环境中,使用EGb的MRC-5细胞生长绝对数明显比空白对照多,且呈现EGb浓度越高,细胞绝对数越多、不同时期ROS含量越低、衰老细胞越少的趋势。结论银杏叶提取物对慢性氧化应激下的MRC-5有保护及抗衰老作用。Objective To explore the antioxidative and anti-aging effect of extract of ginkgo biloba （EGb） on human fibroblasts in vitro. Methods Human embryo pulmonary fibroblasts （ MRC-5 ） were cultured under circumstance of normoxia （ oxygen concentration 21% ） and hyperoxia （oxygen concentration 40% ） respectively and treated with different concentrations of EGb （0, 7. 5, 15, 30, 60 μg/ml, respectively）. The well with 400 μmol/L phenyhertbutylnitrone （PBN） served as positive control. The growth curves of MRC-5 were recorded and intracellular content of reactive oxygen species （ROS） of MRC-5 were measured in different conditions and time points. Results As a whole, under normoxia, neither growth curves nor ROS contents were significant different in various concentration of EGb. While in the circumstance of hyperoxia, the absolute number of MRC-5 in wells treated with EGb were greatly higher than those in untreated ones, with a trend of the more EGb given, the better MRC-5 grew, the less intracellular ROS content and the less senescent cells in different time points. Conclusions EGb has a protective and anti-aging effect on MRC-5 fibroblasts under chronic mild oxidative stress in vitro.

关 键 词：银杏液提取物 抗氧化 抗衰老 MRC-5 细胞培养 

分 类 号：R394[医药卫生—医学遗传学]