新疆地区克里米亚刚果出血热病毒M片段的遗传分析  

Genetic analysis of M gene of Crimean-Congo hemorrhagic fever virus isolate in Xinjiang

作　　者：孙素荣[1] 胡俊[1] 张渝疆[2] 孟卫卫[2] 冯崇慧[2] 戴翔[2] 张富春[1] 解燕乡[3] 杭长寿[3] 

机构地区：[1]新疆大学生命科学与技术学院分子生物学重点实验室,新疆生物资源基因工程重点实验室,乌鲁木齐830046 [2]新疆疾病预防控制中心,830002 [3]中国疾病预防控制中心病毒病预防控制所

出　　处：《中华微生物学和免疫学杂志》2007年第6期560-564,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家"十五"科技攻关项目(编号:2003BA712A08-03)

摘　　要：目的为进一步了解克里米亚刚果出血热病毒(CCHFV)M基因的特征,对新疆地区4株克里米亚刚果出血热病毒分离株亚东璃眼蜱的部分M片段核苷酸序列进行测定及分析。方法从感染CCHF病毒的鼠脑中提取RNA,应用逆转录聚合酶链反应扩增,测定CCHF病毒的部分M片段核苷酸序列,与GenBank中的已登录的部分CCHF病毒M基因的同源序列进行比较分析和种系进化分析。结果经测序得到了4株病毒的3962～4385 nt位点的424 bp核苷酸序列(位点依据IbAr10200的M基因序列),该序列共编码139个氨基酸。前3株病毒序列有相当高的核苷酸同源性(99.5%～99.8%),将所得到的序列与已发表的中国、尼日利亚、巴基斯坦、乌兹别克斯坦、塔吉克斯坦和俄罗斯病毒株相比,中国株之间的核苷酸同源性为78.1%～99.8%,而非中国株则为42.9%～94.8%。但它们所编码的氨基酸序列变异不大,同源性非常高。系统发生树表明,所有的毒株被分为5个进化支(所比较的M基因长度为3962～4385 nt核苷酸),来自新疆南部和东部的YT05099、YLD4041和YL05035共处于同一分支。结论相比于新疆其他分离株,YT05099、YL04041和YL05035的部分M基因特征有极高的相似性。新疆株CCHF病毒M基因与中东和远东病毒株之间有较近的亲缘关系。Objective To obtain a better understanding of the molecular characteristic of the medium genome segment （M segment） of Crimean-Congo hemorrhagic fever virus （CCHFV） in Xinjiang. Methods RNA was extracted from the brain of mice infected by CCHFV. Reverse transcriptase PCR （RT-PCR） was performed, the sequence of M segment was examined and compared with those of CCHF virus in GenBank. Results The sequences of M segment with a length of 424 bp（3962-4385 nt, reference stain IbAr10200） encoding 139 amino acids were found in 4 samples, among which, 3 shares high homology（99.5%-99.8% ）. When compared with other available published sequences of M segment in strains from China, Nigeria, Pakistan, Uzbekistin, Tajikistan and Russia, the partial M segment from Xinjiang strains revealed a close relatedness among isolate in China（nucleotide sequence homologies, 78.1%-99.8%）, and differed significantly from those in other regions of the world（nucle- otide sequence homologies, 42.9%-94.8% ）. But the coded amino acid sequence is almost the same. Phylogenetie analysis found that, based on the 424 bp length nucleotide sequence of the M genome segnent, all the known strains can be divided into five genetic groups, whereas YT05099, YL04041 and YL05035 strains from the southern and eastern of Xinjiang form a separate group. Conclusion The characteristics of partial M segment of YT05099, YL04041 and YL05035 were similar to the remains of CCHF virus strains regions of Xinjiang. The partial M segmont Xinjinag strain exhibits highest nucleotide and amino acid similarity to the Middle East and Far East virus.

关 键 词：克里米亚刚果出血热病毒 M片段 核苷酸序列 系统发生树 

分 类 号：R373[医药卫生—病原生物学]