抑癌基因PRDM1在肺癌组织中的表达  

Expression of tumor suppressor gene PRDMl in lung cancers

作　　者：刘艳艳[1] 杨树军[2] 马杰[3] 王黎[1] 刘元华[1] 陈赛娟[1] 沈志祥[1] 赵维莅[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院血液科上海血液学研究所,200025 [2]河南省肿瘤医院肿瘤内科 [3]河南省肿瘤医院病理科

出　　处：《中华肿瘤杂志》2007年第6期434-436,共3页Chinese Journal of Oncology

基　　金：上海市科学技术委员会国际合作交流资助项目(044107025);上海市青年科技启明星计划资助项目(05QMX1429)

摘　　要：目的探讨抑癌基因PRDM1在肺癌组织中的表达情况及其意义。方法45例肺癌患者中,鳞状细胞癌20例、腺癌15例、小细胞肺癌10例。用免疫组织化学方法,研究肺癌石蜡包埋组织中PRDM1蛋白的表达情况;激光微切割技术捕捉冰冻肺癌组织中的肿瘤细胞,抽提RNA、采用逆转录聚合酶链式反应(RT-PCR)技术研究PRDM1基因表达;Western blot技术研究冰冻肺癌组织中PRDM1蛋白表达。结果(1)免疫组织化学结果表明鳞状细胞癌、腺癌、小细胞肺癌患者的PRDM1蛋白阳性率分别为90.0%(18/20)、13.3%(2/15)和0(0/10),统计学分析显示,PRDM1蛋白主要在鳞状细胞癌患者中表达(P<0.01)。(2)激光微切割技术联合RT-PCR证实,PRDM1基因特异表达于鳞状细胞癌患者中,腺癌和小细胞肺癌中未发现其基因表达。进一步研究发现,鳞状细胞肺癌患者中,PRDM1基因的DNA结合区表达缺陷。Western blot在鳞状细胞肺癌组织中检测出约70 000的异常PRDM1蛋白。结论PRDM1在鳞状细胞肺癌中优势表达,可作为肺癌诊断的新的肿瘤标志;鳞状细胞肺癌中PRDM1在转录和蛋白水平上均存在异常,失去了抑癌基因的正常功能,可能成为新的研究治疗靶点。Objective To determine the expression of tumor suppressor gene PRDM1 in lung cancers. Methods Forty-five cases were enrolled in this study, including squamous cell carcinoma （20 cases） , adenocarcinoma （15 cases） , and small cell cancer （10 cases）. PRDM1 protein was detected in paraffin-embedded tissue by immunohistoehemistry. Tumor cells in lung cancers were further selected by laser microdissection for RT-PCR analysis. PRDM1 protein in frozen tissue was also detected by Western blot. Results （ 1 ） PRDM1 protein was found in paraffin-embedded tissues in 90. 0% （ 18/20 ） of squamous cell carcinoma, 13.3% （2/15） of adenocarcinoma, and 0 （0/10） small cell lung cancer. Squamous cell carcinoma predominantly expressed PRDM1 protein （ P 〈 0. 01 ）. （ 2 ） Gene product of PRDM1 DNA binding region was not found in microdissected tumor cells, but an abnormal PRDM1 protein about 70 KD was detected simultaneously in whole tumor tissue. Conclusion PRDM1 may be considered as a specific biomarker in pulmonary squamous cell carcinoma. The abnormal PRDM1 expression both at transcriptional and protein levels indicated that this tumor suppressor gene lost its function, which may become a new target in the strategy of treatment for lung cancers.

关 键 词：抑癌基因PRDM1 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]