PTEN抑癌基因对A549肺癌细胞辐射敏感性的影响  

The Effect of Exogenous Tumor Suppressor PTEN on Radiosensitivity of Lung Cancer A549 Cells

作　　者：赵正源[1] 田丰[1] 刘锟[1] 程庆书[1] 高坤祥[1] 

机构地区：[1]第四军医大学唐都医院胸外科,陕西西安710038

出　　处：《实用放射学杂志》2007年第6期835-838,共4页Journal of Practical Radiology

摘　　要：目的评价外源性PTEN抑癌基因对人A549肺癌细胞辐射敏感性的影响。方法体外培养A549肺癌细胞株,用脂质体介导法将PTEN导入A549肺癌细胞系,以空载体转染细胞作为对照。然后用MTT法和克隆形成实验测定2种癌细胞对^(60)Co-γ射线的敏感性。并检测肿瘤细胞PKB的表达。结果相同剂量照射下,A549-pBP-PTEN细胞存活与A549细胞相比明显减少,治疗指数分别为1.69;转染PTEN对肿瘤细胞PKB的表达产生抑制。结论外源性野生型PTEN抑癌基因能增强A549肺癌细胞对γ射线的敏感性。Objective To investigate the Effect of exogenous tumor suppressor PTEN on radiosensitivity of lung cancer A549 cells. Methods Retrovirus expression vector containing wildtype PTEN gene or control vector were introduced into the A549 cells by liposome, and PTEN transfected ceils were named as A549 - pBP - PTEN. The radiosensitivity of A549 cell line to ^60Co - γ irradiation was determined by MTT assay and clone formation assay. The activity of PKB in tumor cells was detected. Results In comparison with the control cells, the PTEN transfected cells revealed more sensitivity to ^60Co - γ irradiation. The activity of PKB was inhibited by Transfection of PTEN. Conclusion Introduction of PTEN gene into A549 cell line can enhance the cells sensitivity to irradiation.

关 键 词：基因 肺癌 射线敏感性 

分 类 号：R734.2[医药卫生—肿瘤] R814.49[医药卫生—临床医学]