上皮性卵巢癌DNA含量、细胞核形态及其对患者预后价值的探讨  

Study on cellular DNA content and morphometry assessment of epithelial ovarian carcinomas by image cytometry.

作　　者：李红霞 汤春生[1,2] 姜玉菲 

机构地区：[1]洛阳医专附属医院 [2]山东省立医院

出　　处：《现代妇产科进展》1997年第2期107-110,共4页Progress in Obstetrics and Gynecology

摘　　要：目的：探讨ＤＮＡ含量、核形态参数对预测上皮性卵巢癌患者预后的价值。方法：应用计算机图像分析系统测定原发性上皮性卵巢癌３２例的细胞核ＤＮＡ含量、倍体水平、面积、周长及形状因子５个参数。结果：卵巢癌临床晚期及转移病例的ＤＮＡ含量明显增高，与早期及无转移组相比，差异均有显著性（Ｐ＜０．０２５）。６３％为异倍体肿瘤，３７％为二倍体肿瘤。组织学Ⅰ、Ⅱ、Ⅲ级的异倍体率分别为３８％、５７％、９０％，呈递增趋势。Ⅰ级与Ⅲ级相比，差异有显著性（Ｐ＜０．０５）。腹水阳性组及转移组的异倍体率明显增加，分别为７３％及７６％。ＰＣＮＡ阳性组及阴性组的核形状因子差异有显著性（Ｐ＜０．０５）。核面积和周长在诸预后因素间差异无显著性（Ｐ＞０．０５）。结论：细胞核ＤＮＡ含量和倍体水平是影响卵巢癌患者预后的重要因素；核形状因子亦可作为评估肿瘤生物学行为的一项指标。Objective To determine the prognostic value of the cellular DNA content,nuclear morphological parameters in patients with epithelial ovarian carcinomas Methods The Feulgen stained sections of 32 cases with epithelial ovarian carcinomas were examined for cellular DNA content,DNA ploidy,nuclear area,nuclear perimeter and nuclear shape factor by using a computerized image analysis system. Results DNA contents were higher in advanced stage and tumor metastasis groups( P <0.025).63% cases were aneuploid while the 37% cases were diploid.The increased aneuploid rate deviated significantly from grade Ⅰ,grade Ⅱ to grade Ⅲ(38%,57%,90%,respectively), P <0.05.The aneuploid rates in ascites and tumor metastasis groups were significantly increased(73% and 76%,respectively), P <0.05.Nuclear shape factor was associated with proliferating cell nuclear antigen(PCNA) positive expression( P <0.05) Conclusions The results show that 60th DNA content and DNA ploidy are significant prognostic factors in patients with ovarian cancer.The nuclear shape factor also may be used as an indicator for the biological behavior of tumor aggression

关 键 词：卵巢肿瘤 核形态 DNA 预后 

分 类 号：R737.310.7[医药卫生—肿瘤]