肝硬化基础上大鼠肝癌模型的建立  

Establishment of liver cancer model based on liver cirrhosis in rats

作　　者：阎丽[1] 柳婧美[2] 乔伟[3] 宋健[1] 王群英[1] 钟慧闽[1] 

机构地区：[1]解放军第401医院消化内科,山东青岛266071 [2]第四军医大学西京医院消化病研究所,陕西西安710033 [3]西安市铁路中心医院肝胆外科,陕西西安710054

出　　处：《第四军医大学学报》2007年第13期1181-1182,共2页Journal of the Fourth Military Medical University

摘　　要：目的:探讨建立一种肝硬化基础上大鼠肝癌模型的方法.方法:60只雄性SD大鼠随机分为两组,对照组和实验组,按60mL/LCCl4和100mL/L食用白酒的方法诱导肝癌模型.观察两组大鼠不同时期质量变化,死亡率,肝假小叶形成率及肝癌结节发生率.结果:诱导16wk后,对照组大鼠质量明显增加,无死亡,无肝硬化,无肝癌形成.实验组大鼠在实验过程中质量增长缓慢,2wk后假小叶形成率90%,继续诱导4wk后癌结节形成率40%.实验过程中大鼠的死亡率为10%.结论:CCl4和食用白酒可成功诱导大鼠肝硬化模型,延长诱导时间可产生一定数量的肝癌模型.AIM： To investigate one method of establishing liver cancer model based on liver cirrhosis in rats. METHODS： Sixty male SD rats were randomly divided into 2 groups, control group and experimental group. The former （ n = 30） were injected sc with olive oil solution （3 μL/g） and fed ordinary diet and water; the latter （n = 30） were treated with CCl4 and drinkable alcohol to induce liver cancer medel. The changes of body mass, mortality, formation rates of liver cirrhosis false lobe and liver cancer in the 2 groups were observed. RESULTS： After being induced with above methods for 6 weeks, in control group, the body mass of rats increased significantly but the mortality, liver cirrhosis false lobe, liver cancer were not observed. In experimental group, the body mass of rats increased slowly and the formation rate of liver cirrhosis false lobe was 90% at the end of 12 weeks; after induction for 4 weeks, formation rate of liver cancer was 40% ; the mortality of the rats was 10% during the experiment. CONCLUSION ： Liver cirrhosis model of rat can be successfully induced by CC14 and drinkable alcohol and some liver cancer models can be induced by prolonged induction time.

关 键 词：肝硬化 肝肿瘤 动物模型 四氯化碳 

分 类 号：R735.4[医药卫生—肿瘤]