大鼠海马神经元中μ阿片受体、CCK受体的表达及慢性吗啡作用对其表达的影响  

Expressions of μ opioid receptor and CCK receptor in rat primary hippocampal neurons and effect of chronic morphine exposure on them

作　　者：闫玉仙[1] 呼文亮[2] 丛斌[1] 马春玲[1] 倪志宇[1] 牛增强[1] 余磊[1] 

机构地区：[1]河北医科大学法医系,河北石家庄050017 [2]武警医学院中心实验室,天津300162

出　　处：《第四军医大学学报》2007年第13期1214-1217,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30672355)

摘　　要：目的:研究MOR-1,CCK-AR,CCK-BR在海马神经元的分布和吗啡对其受体的影响.方法:用激光共聚焦扫描技术,以神经元特异性标记抗体NF-200对原代海马神经元进行鉴定,采用特异性的MOR-1,CCK-AR,CCK-BR的抗体观察观察它们在海马神经元上的表达.选用雄性Wister大鼠,用剂量递增法皮下注射盐酸吗啡,对照组以同样的方法给予生理盐水后,取出大鼠海马组织用流式细胞技术检测不同时间吗啡作用于海马神经元时上述受体的表达.结果:海马神经元上同时表达MOR-1,CCK-AR及CCK-BR;给予吗啡后2～4d,MOR-1在海马神经元上的表达较对照增高(P<0.05),5d时,MOR-1的表达显著下降(P<0.01);随吗啡作用时间延长,CCK-AR和CCK-BR在海马神经元的表达呈上调趋势,且CCK-BR的表达高于CCK-AR(P<0.01).结论:海马神经元上同时存在MOR-1,CCK-AR和CCK-BR,吗啡作用时间越长,MOR-1表达越弱,而CCK-AR和CCK-BR表达越强.AIM ： To investigate the distributions of μ opioid receptor （MOR-1）, cholecystokinin A receptor （CCK-AR） and cholecystokinin B receptor （CCK-BR） in rat primary hippocampal neurons and the effect of morphine on them. METHODS： The primary hippocampal neurons were identified by fluorescence labeled NF-200, and observed under laser scanning confocal microscope. MOR-1, CCK-AR and CCK-BR specific antibodies were applied to test three receptors co-distribution in hippocampal neurons. Sixty male Wistar rats were injected s. c with morphine-HCl gradually increasing doses. Control rats were treated with saline under the same condition. The acute isolated hippocampal neurons were prepared to examine the expressions of MOR-1, CCK-AR and CCK-BR by flow cytometry. RESULTS： CCK-AR, CCK-BR and μ opioid receptors expressed simultaneously in hippocampal neurons. The fluorescence intensity of μ opioid receptor in hippocampal neurons of the 2 - 4 d groups was remarkably higher than that of control group （P〈0.05）. The 5th day MOR- 1 expression decreased remarkably （ P 〈 0.01 ）. The expressions of CCK-AR, CCK-BR receptors were up-regulated in hippocampal neurons in a time-dependent manner during morphine treatment （ P 〈 0.01 ）. The expression of CCK-B receptor was stronger than that of CCK-A receptor （P 〈 0.01 ）. CONCLUSION： MOR-1 CCK-AR and CCK-BR receptor coexist in hippocampal neurons.Continued exposure to morphine initially increases MOR-1 expression, followed by decreased MOR-1 expression. CCK-AR, CCK- BR receptors increases slowly in a time-dependent manner during morphine treatments.

关 键 词：阿片受体 CCK-A受体 CCK-B受体 吗啡依赖 原代海马神经元 激光共聚焦 流式细胞术 

分 类 号：R747.205[医药卫生—神经病学与精神病学]