Science Letters:Simultaneous determination of fluoride, chloride, sulfate, phosphate, monofluorophosphate, glycerophosphate, sorbate, and saccharin in gargles by ion chromatography  

作　　者：ZHANG Yan-zhen ZHOU Yan-chun LIU Li ZHU Yan 

机构地区：[1]Department of Stomatology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China [2]Department of Stomatology, Hospital of Zhejiang University, Hangzhou 310027, China [3]Affiliated Stomatology Hospital School of Medicine, Zhejiang University, Hangzhou 310006, China [4]Department of Chemistry, Zhejiang University, Hangzhou 310028, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2007年第7期507-511,共5页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation of China (Nos. 20375035 and 20527005);the Natural Science Foundation of Zhejiang Province, China (Nos. Z404105 and Y405415);the Science and Technology Department of Zhejiang Province, China (No. 2007C33016)

摘　　要：Simple, reliable and sensitive analytical methods to determine anticariogenic agents, preservatives, and artificial sweeteners contained in commercial gargles are necessary for evaluating their effectiveness, safety, and quality. An ion chroma-tography (IC) method has been described to analyze simultaneously eight anions including fluoride, chloride, sulfate, phosphate, monofluorophosphate, glycerophosphate (anticariogenic agents), sorbate (a preservative), and saccharin (an artificial sweetener) in gargles. In this IC system, we applied a mobile phased gradient elution with KOH, separation by IonPac AS18 columns, and suppressed conductivity detection. Optimized analytical conditions were further evaluated for accuracy. The relative standard deviations (RSDs) of the inter-day's retention time and peak area of all species were less than 0.938% and 8.731%, respectively, while RSDs of 5-day retention time and peak area were less than 1.265% and 8.934%, respectively. The correlation coefficients for targeted analytes ranged from 0.999 7 to 1.000 0. The spiked recoveries for the anions were 90%~102.5%. We concluded that the method can be applied for comprehensive evaluation of commercial gargles.Simple, reliable and sensitive analytical methods to determine anticariogenic agents, preservatives, and artificial sweeteners contained in commercial gargles are necessary for evaluating their effectiveness, safety, and quality. An ion chromatography （IC） method has been described to analyze simultaneously eight anions including fluoride, chloride, sulfate, phosphate, monofluorophosphate, glycerophosphate （anticariogenic agents）, sorbate （a preservative）, and saccharin （an artificial sweetener） in gargles. In this IC system, we applied a mobile phased gradient elution with KOH, separation by IonPac AS18 columns, and suppressed conductivity detection. Optimized analytical conditions were further evaluated for accuracy. The relative standard deviations （RSDs） of the inter-day＇s retention time and peak area of all species were less than 0.938% and 8.731%, respectively, while RSDs of 5-day retention time and peak area were less than 1.265% and 8.934%, respectively. The correlation coefficients for targeted analytes ranged from 0.999 7 to 1.000 0. The spiked recoveries for the anions were 90%-102.5%. We concluded that the method can be applied for comprehensive evaluation of commercial gargles.

关 键 词：Ion chromatography ANIONS Gargles MONOFLUOROPHOSPHATE SORBATE SACCHARIN 

分 类 号：R78[医药卫生—口腔医学]