多肽铬螯合物对糖尿病小鼠肝脏蛋白质表达影响的研究  

Study on Effect of Polypeptide Chromium Chelate on Hepatic Protein Expression in Diabetic Mice

作　　者：刘安军[1] 马艳弘[1] 张国蓉[1] 王秀丽[1] 陈影[1] 孟娜娜[1] 孙海波[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《现代生物医学进展》2007年第7期961-963,968,共4页Progress in Modern Biomedicine

基　　金：天津科技大学引进人才科研启动基金(0200038);国家自然科学基金项目(20576101)

摘　　要：目的:研究多肽铬螯合物对糖尿病小鼠肝脏蛋白质表达的影响,探讨其治疗糖尿病的机理。方法:通过腹腔注射四氧嘧啶建立糖尿病小鼠模型。将小鼠分为正常组模型组和胶铬组,胶铬组以灌胃方式加入多肽铬螯合物;以光镜及HE染色观察三组小鼠肝脏形态及组织学的变化,采用SDS-PAGE实验和非SDS-PAGE检测三组小鼠肝脏蛋白质表达。结果:光镜及组织学观察结果显示多肽铬螯合物可以有效地减轻四氧嘧啶对肝细胞造成的损伤。模型组肝脏25kDa-35kDa之间的某种蛋白表达升高而多肽铬螯合物可以降低此种蛋白的表达,初步推断这种蛋白质为SOD。结论:多肽铬螯合物能够通过降低四氧嘧啶造成的肝脏中抗氧化有关的蛋白质代偿性升高而起到保护肝脏的作用,是一种新型的治疗糖尿病所致的肝损伤的活性物质。Objective： To study the effect of polypeptide chromium chelate on hepatic protein expression in diabetic mice. Methods： Diabetic mouse model was set up by i.p. injection of alloxan, the mice were divided into normal group （NG）, model group （MG） and polypeptide chromium group （PCG）. Polypeptide chromium chelate was added into PCG by i.g. method. The changes of hepatic morphology and histology in the three groups were observed by HE staining under microscope, and hepatic protein expression was examined by SDS-PAGE and non SDS-PAGE experiments in the three groups. Results： The results showed that polypeptide chromium chelate could effectively alleviate the hepatocytes lesion induced by alloxan. Expression of a kind of protein between 25kDa and 35kDa in model group increased and polypeptide chromium chelate could decrease its expression, this protein was deduced to be SOD initially. Conclusion： Polypeptide chromium chelate could decrease the compensatory elevation of the antioxidant related protein in the liver induced by alloxan and perform the hepatoprotective function, which is a novel active substance in treatment of hepatic injury induced by diabetes.

关 键 词：多肽铬螯合物 四氧嘧啶 糖尿病 四氮唑蓝染色 

分 类 号：R587.1[医药卫生—内分泌]