西索米星分批发酵过程动力学模型  

Kinetic models of sisomicin in a batch fermentation process

作　　者：陈剑锋[1] 邵敬伟[1] 张元兴[2] 陈浩[1] 郭养浩[1] 

机构地区：[1]福州大学生物科学与工程学院,福建福州350002 [2]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《福州大学学报（自然科学版）》2007年第3期475-480,共6页Journal of Fuzhou University(Natural Science Edition)

基　　金：福建省自然科学基金资助项目(C0210007;B9810007);福建省教育厅科技基金资助项目(K20011)

摘　　要：采用伊尼奥小单孢菌F003为产生菌的西索米星发酵过程具有典型的非生长耦联特征,西索米星浓度大于0.50g/L将明显抑制产物合成.建立了符合不同时期微生物代谢特性的西索米星分批发酵动力学模型,并对模型参数进行了估计和回归,模型拟合偏差平方和≤6%,模型能很好地描述西索米星发酵过程菌体的生长、底物的消耗和产物的合成.菌体生长期动力学模型参数μm、Ks、Kx、m分别为：0.058 h-1、4.046 g/L、0.433 g/g、0.0001 g/（g·h）;产物合成期动力学模型参数μKx、K2、N、Yx、Yp、m分别为：0.058 h^-1、60.556 g/g、1.198 g/（g·h）、0.606、0.090 g/g、0.095 g/g、0.00173 g/（g·h）.验证实验表明,所建立的模型能较好地预测初始淀粉浓度为50.0～70.0 g/L时西索米星的发酵过程特性.The sisomiein production was found to be non-associated with the cell growth of mieromonospora inyoensis F003, and sisomiein above 0.5 g/L was inhibitive to its biosynthesis. On the basis, the unstructured kinetic models for the batch fermentations of mieromonospora inyoensis F003 were constructed, and the model parameters were estimated and regressed by MathCAD. The model simulation average deviation was less than 6.0%. The cell growth, substrate consumption and metabolite production were described well by the model. The estimated values of the model parameters during exponential growth phase were such as ：μm = 0. 058 h - z, Ks = 4. 046 g/L, Kx = 0. 433 g/g, m = 0. 000 1 g/（ g · h） ; The estimated values of model parameters during sisomiein biosynthesis phase were as follows μm = 0.058 h^ -1, Kx = 60. 556 g/g, K2 = 1. 198 g/（ g · h）, N = 0.606, Yx = 0. 090 g/g, Yp = 0.095 g/g, m =0.001 73 g/（g · h）. The model could predict the fermentation characteristics of micromonospora inyoensis F003 at the initial starch concentration of 50.0 -70.0 g/L.

关 键 词：伊尼奥小单孢菌F003 西索米星 分批发酵 动力学模型 

分 类 号：TQ465.2[化学工程—制药化工]