过表达Notch1对肿瘤细胞增殖的影响  

Effect of Notch1 overexpression on proliferation of cancer cell lines

作　　者：俞海[1] 葛盛芳[1] 卢健[1] 钱关祥[1] 

机构地区：[1]上海交通大学基础医学院生物化学与分子生物学教研室,上海200025

出　　处：《上海交通大学学报（医学版）》2007年第3期289-292,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家重点基础研究发展项目("九七三"项目;2004CB518804);上海市科委基金(04JC14041)~~

摘　　要：目的通过在肿瘤细胞内过表达Notch1胞内段活性部分,模拟细胞Notch途径的上调,检测细胞增殖状态的变化。方法利用包含Notch1胞内段的逆转录病毒感染实验细胞。通过CBF-1报告载体检测细胞内Notch信号的激活程度,MTS法检测细胞增殖情况,并对细胞的周期分布进行检测。结果Notch1胞内段的表达引起Notch信号在不同细胞中的上调,并引起Hela和HepG2细胞的增殖抑制和BGC-823细胞的增殖加速,而对Chang细胞的增殖没有明显影响。结论Notch信号的上调能引起不同肿瘤细胞增殖状态的变化,对Notch在肿瘤基因治疗中的应用具有重要意义。Objective To upregulate Notch signaling in cancer cells by overexpression of active part of Notchl and to examine the proliferation of the ceils. Methods Four cancer cell lines were infected with retrovirus recombined with sequence encoding active part of Notchl. CBF-1 reporter plasmid was used to detect Notch signaling and proliferation assay was carried out by MTS method. Cell cycle analysis was synchronously conducted. Results The overexpression of the active part of Notchl induced upregulation of Notch signaling, led to growth inhibition in Hela and HepG2 cell lines and growth boost in BGC-823 cell lines, while had no effect on Chang cell lines. Conclusion The upregulation of Notch signaling can exert various effects on different cancer cell lines which is critical to the gene therapy for cancers.

关 键 词：NOTCH 肿瘤 基因治疗 

分 类 号：R730.2[医药卫生—肿瘤]