安徽汉族免疫性不育症与HLA-DQA1基因的相关性研究  

Study on the Association Between the HLA-DQA1 Alleles with Immunologic Infertility of Han Nationality in Anhui Province

作　　者：杜华荣[1] 王望九[1] 余元勋 戴宁[3] 梁文珍[3] 黄震[1] 申国明[1] 马宗华[1] 陈永华[1] 张凯[1] 余荣娇[1] 洪燕[1] 

机构地区：[1]安徽中医学院,安徽合肥230038 [2]安徽省医学遗传研究所,安徽合肥230001 [3]安徽中医学院第一附属医院,安徽合肥230031

出　　处：《生物技术通讯》2007年第2期240-242,共3页Letters in Biotechnology

基　　金：安徽省自然科学基金项目(03043003);安徽省教育厅科研基金项目(2000J1146)

摘　　要：目的:探讨抗精子抗体阳性的免疫性不育症患者与HLA-DQA1基因的相关性。方法:采用聚合酶链反应-序列特异性引物(PCR-SSP)技术,对50例抗精子抗体阳性的免疫性不育症患者和60例正常健康者的HLA-DQA1基因进行分型研究。结果:2组均未发现DQA1I0104和DQA1I0302两种基因型。免疫性不育症组DQA1I0401等位基因频率明显高于正常对照组(RR=5.5,P<0.05)。结论:DQA1I0401可能是安徽汉族免疫性不育症的易感基因。Objective： To explore the association between the HLA-DQA 1 alleles with Immun-ologic infertility of Han nationality in Anhui Province. Methods： Polymerase chain reaction sequence specific prime （PCR-SSP） was used to type HLA-DQA1 subregion in 50 patients with immunologic infertility and 60 unrelated healthy control of Han nationality in Anhui Province. Results： The HLA DQA1＊O104 and DQA1＊0302 alleles in both group were not found. The allelic frequency of HLA-DQA 1＊0401 was significantly increased in immunologic infertility patients than in the control（RR=5.5, P〈 0.05）. Conclusion： It suggested that the HLA-DQA1＊0401 allele may be suscep-tible gene for immunologic infertility in Han nationality in Anhui Province.

关 键 词：抗精子抗体 免疫性不育症 人类白细胞抗原 等位基因 遗传易感性 

分 类 号：Q75[生物学—分子生物学] Q347