绿脓杆菌外毒素A对兔角膜基质细胞的毒性作用  

Toxic effect of exotoxin A of Pseudomonas aeruginosa on keratocytes

作　　者：郝继龙[1] 王菲[2] 周鸿雁[1] 赵文霞[1] 谷树严[1] 

机构地区：[1]吉林大学中日联谊医院眼科,吉林长春130033 [2]黑龙江省哈尔滨市第一医院眼科,黑龙江哈尔滨150001

出　　处：《吉林大学学报（医学版）》2007年第3期488-490,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅资助课题(20010524)

摘　　要：目的:探讨绿脓杆菌外毒素A兔对角膜基质细胞的毒性作用。方法:含兔角膜基质细胞(1×105.mL-1)三维胶原凝胶表面添加提纯的不同浓度绿脓杆菌外毒素A(0.1、1.0和10 mg.L-1)后,放入无血清MEM培养液中37℃培养24 h,采用光学显微镜观察培养的兔角膜基质细胞形态学变化,采用酶联免疫吸附法检测损伤的兔角膜基质细胞释放的乳酸脱氢酶(LDH)含量。结果:添加不同浓度绿脓杆菌外毒素A(0.1、1.0和10 mg.L-1)各组兔角膜基质细胞释放的LDH,与不添加绿脓杆菌外毒素A组比较,差异均有显著性(P<0.01)。添加不同浓度绿脓杆菌外毒素A各组较不添加绿脓杆菌外毒素A组,角膜基质细胞形态发生变化,细胞由长纺锤形变为圆形,细胞伪足减少。结论:绿脓杆菌外毒素A,破坏角膜基质细胞,对兔角膜基质细胞毒性作用有剂量依赖性。Objective To study the toxic effect of exotoxin A of Pseudomonas aeruginosa on keratocytes. Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A （0.1, 1.0, 10 mg · L^-1 ）, cultivated for 24 h at 37℃, the change of keratocytes in morphology was observed under the light microscope, and the amount of lactate dehydrogenase （LDH） was measured by enzyme linked immunosorbent assay （ELISA）. Results The LDH contents in different concentrations （0.1, 1.0, 10 mg·L^-1） of exotoxin A groups were higher than that in the group without exotoxin A （P〈0.01）. The normal keratocytes were long atractoid. When keratocytes were incubated in the presence of different concentrations of exotoxin A, they changed into cycloid . Conclusion Exotoxin A of Pseudomonas aeruginosa can injury keratocytes and its effect is in a dose-dependent manner.

关 键 词：假单胞菌 铜绿 角膜基质细胞 外毒素类 乳酸脱氢酶 

分 类 号：R772.2[医药卫生—眼科]