Synphilin-1寡核苷酸对帕金森病酪氨酸羟化酶表达的影响  

THE INFLUENCE OF Synphilin-1 OLIGONUCLEOTIDE ON TYROSINE HYDROXYLASE IN PARKINSON DISEASE

作　　者：谢安木[1] 薛莉[2] 刘焯霖[3] 

机构地区：[1]青岛大学医学院附属医院神经内科,山东青岛266003 [2]青岛大学医学院附属医院康复科 [3]中山大学第一附属医院神经科

出　　处：《青岛大学医学院学报》2007年第3期199-201,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省中青年科学家基金(2005BS03021)

摘　　要：目的探讨Synphilin-1对帕金森病(PD)酪氨酸羟化酶(TH)表达的影响。方法50只SD大鼠随机分成5组,每组10只。PD组大鼠右侧黑质注入6-羟多巴3μL制作PD模型,反义组、同义组、错义组分别注入Synphilin-1反义寡核苷酸、同义寡核苷酸、错义寡核苷酸各3μL,正常对照组右侧黑质注入2 g/L抗坏血酸生理盐水。取鼠的术侧脑黑质制作切片,利用免疫组化方法检测Synphilin-1表达,利用免疫组化、Western blot、原位杂交方法检测TH的表达。结果与正常对照组比较,PD组Synphilin-1蛋白表达显著增加,TH蛋白和TH mRNA表达显著减少;与PD组比较,Synphilin-1反义组TH蛋白和TH mRNA表达显著增多。结论Synphilin-1可能通过影响TH的表达对帕金森病的发病机制产生影响。Objective To investigate the effect of synphilin-1 on tyrosine hydroxylase （TH） expression of Parkinson di sease. Methods Fifty healthy male Sprague-Dawley rats were divided evenly into five groups. PD group. PD models were made by unilateral stereotaxic infusion of 3 μL of 6-hydroxydopamine （6-OHDA） in right substantia nigra. Antisense group, sense group and missense group were given antisense, sense, and missense oligonucleotides respectively. Control group： natrium saline was injected into right substantia nigra. The expression of synphilin -1 protein was detected by immunohistochemistry, and expression of TH protein and mRNA immunohistochemistry, western blot, and in situ hybridization. Results Compared with control group group, the expression of synphilin- 1 in PD significantly increased, and the expression of TH decreased. Compared with PD group, the expression of TH protein and TH mRNA in antisense group significantly increased. Conclusion Synphilin -1 may affect the pathogenesis of PD by affecting the TH expression.

关 键 词：SYNPHILIN-1 酪氨酸单氧化酶 帕金森病 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]