抗登革病毒因子的研制  

Preparation of Antiviral Factor Against Dengue Virus

作　　者：陈德元[1] 饶颐年 谢名荣[1] 郭秀兰 王国斌[1] 

机构地区：[1]广州医学高等专科学校,广州510315 [2]广州军区军事医学研究所

出　　处：《中国人兽共患病杂志》1997年第2期7-9,共3页Chinese Journal of Zoonoses

摘　　要：登革病毒具有抗体依赖性病毒增强作用，故目前尚无有效的疫苗供临床使用。本实验用革登病毒免疫鸡、从高敏蛋黄中提取抗登革病毒因子，通过登革病毒乳鼠动物模型，观察该因子对动物的免疫保护作用。结果表明：用致死量的登革病毒攻击乳鼠，实验组的病死率为32％，因子对照组的病死率为100％，两组差异非常显著（P＜0．01）。实验组病死扣鼠平均存活时间为9．17±2．04天，因子对照组为7．82±0．87天，两组差异显著（P＜0．05），显示出良好的紧急预防与治疗作用。该因子可能含有一些介导细胞免疫的小分子物质，而不含有介导体液免疫的抗体，故既能避免抗体依赖性病毒增强作用，又能通过细胞免疫杀灭和清除病毒，从而具有较好的紧急预防和治疗作用，可能为登革热的防治提供一条可行的途径。Development of dengue virus vaccine has been hindered by the fact that antibody might enhance growth of dengue virus in macrophage cells. In the experiment,we immunized.chickens with dengue virus,extracted antiviral factor from egg yolk of eggs laid by highly-sensitized chickens. Immunoprotection against experimental infection with fatal dose of dengue virus in newborn-mice was fourid. The results showed that the case fatality rate of the experimantal groups (32 % ) was significantly lower than those of the control groups (100%, P<0. 01 ). The survival period of infected mice in the experimental groups (9. 17±2. 04 days )was longer than that in the contral groups (7. 82±0. 87 days, P<0. 05 ). The results suggested that the factor had definic immunoprotecti0n against dengue fever. The factor were probably low-molecular-weight antiviral substances mediating cellular immunity.As the factor didn't contain antibody mediating humoral itnmunity,it could avoid the effect of antibody dependent enhancement. The antiviral factor might provide a practical way of prevention and treatment of dengue fever.

关 键 词：登革热病毒 转移因子 

分 类 号：R512.805[医药卫生—内科学] R392－33[医药卫生—临床医学]