低温驯化锯缘青蟹鳃抗氧化防护、ATPase及膜脂肪酸组成变化  

CHANGES OF ANTIOXIDANT DEFENSES,ATPase ACTIVITY AND CELL MEMBRANE FATTY ACID COMPOSITION IN GILL OF SCYLLA SERRATA UNDER LOW TEMPERATURE ACCLIMATION

作　　者：孔祥会[1] 王桂忠[1] 李少菁[1] 

机构地区：[1]厦门大学海洋与环境学院近海海洋环境科学国家重点实验室

出　　处：《水生生物学报》2007年第1期59-66,共8页Acta Hydrobiologica Sinica

基　　金：国家863重大专项(2002AA603013)资助

摘　　要：锯缘青蟹(Scylla serrata)低温环境下生理生化的变化对于理解其低温适应具有重要的意义。本研究中采用生物化学的方法对低温驯化下锯缘青蟹鳃中超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GPX)的活性,脂质过氧化产物丙二醛(malondialdehyde,MDA)含量,4种ATPase(Na+,K+-ATPase,Mg2+-ATPase,Ca2+-ATPase和Ca2+,Mg2+-ATPase)活性及细胞膜脂肪酸组成进行测定。实验结果显示青蟹鳃中抗氧化酶(SOD、CAT和GPX)活性在3个驯化温度下,随驯化温度的降低而升高。SOD活性在5℃和10℃驯化下显著高于对照组(27℃组)(p<0.01);CAT活性在3个驯化温度下均显著高于对照组(p<0.01或p<0.05);GPX活性仅5℃驯化下显著高于对照组(p<0.01)。MDA含量低温驯化下升高,但仅5℃驯化下显著高于对照组(p<0.01)。鳃中4种ATPase活性均是随驯化温度的降低而升高,并且5℃和10℃驯化下均显著高于对照组(p<0.01或p<0.05)。低温驯化下C18:0、C18:1、C18:2、C18:3、C20:5和C22:6等脂肪酸与对照组相比均发生显著性变化(p<0.01或p<0.05),饱和指数∑SFA/∑UFA显著下降(p<0.01或p<0.05)。低温驯化下锯缘青蟹鳃中抗氧酶及ATPase活性升高,说明其具有明显的温度补偿效应,它是对低温适应的一种积极反应。鳃中MDA低温驯化下积累是活性氧自由基未能被及时清除而产生氧化应激的结果。低温下细胞膜脂肪酸饱和指数降低是维持细胞膜执行正常生理功能的需要。Physiological and biochemical changes of Scylla serrata under low temperature condition play an important role in understanding the process of low temperature adaptation. In this study, Superoxi de dismutase （SOD）, Catalase （CAT） and Glutathione peroxidase（GPX） activity, malondialdehyde （MDA） content, 4 ATPase （ Na ^2＋, K^2＋-ATPase, Mg^2＋-ATPase, Ca^2＋-ATPase and Ca^2＋ , Mg^2＋-ATPase） activity, as well as fatty acid composition of cell membrane in gill of S. serrata under low temperature acclimation, were determined by biochemistry methods. The results showed that, for three acclimation temperatures, the activity of antioxidant enzymes （SOD, CAT and GPX） increased gradually with decrease of acclimation temperature. Compared with the control group（27℃ ）, SOD activity increased significantly at 5 ℃ and IO℃ （p 〈 0.01 ） ;CAT activity was higher significantly than that at any of acclimation temperature（p 〈 0.01 or p 〈 0.05） ; GPX activity only increased significantly at 5℃ （p 〈 0.01）. MDA content increased at low temperature, but increased significantly only at 5 ℃ （p 〈 O. O1 ）. 4 ATPase activities in gill also increased gradually with the decrease of the acclimation temperature,and enzyme activity was higher significantly at 5℃and IO℃ than that at 27 ℃. For the content of fatty acids C18 ： O, C18 ： 1, C18 ： 2, C18 ： 3, C20 ： 5 and C22 ： 6 in gill cell membrane under low temperature acclimation, there was the significant difference （p 〈 0.01 or p 〈 0.05） between the acclimation group and the control. Saturation index ∑SFA/∑ UFA in gill under low temperature acclimation significantly decreased （p 〈 0.01, or p 〈 0.05）. The increase of enzyme activity under low temperature indicated that there were obvious compensation effects on temperature in gills of S. serrata,which were the positive responds to adapt to the low temperature. The increment of MDA was caused by oxidant stress resulted from the accumulation

关 键 词：抗氧化效应 ATPASE 脂肪酸 鳃 低温驯化 锯缘青蟹 

分 类 号：Q507[生物学—生物化学]