Synthesis of Novel Phosphorylated Daidzein Derivatives and ESI Investigation on Their Non-Covalent Complexes with Lysozyme  

作　　者：陈晓岚 石晓娜 屈凌波 袁金伟 卢建莎 赵玉芬 

机构地区：[1]Key Laboratory of Chemical Biology and Organic Chemistry of Henan Province, Department of Chemistry, Zhengzhou University, Zhengzhou, Henan 450052, China [2]Anyang Normal University, Anyang, Henan 455002, China [3]The Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China

出　　处：《Chinese Journal of Chemistry》2007年第7期1008-1013,共6页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （Nos. 20132020 and 20175026） and Henan Academic Foundation of Science and Technology.

摘　　要：Daidzein （7,4＇-dihydroxyisoflavone） was phosphorylated by a modified Atherton-Todd reaction. The structures of the five target product, were determined by X-ray, IR, NMR and ESI-MS. Electrospray ionization results show that in the gas phase all the phosphorylated daidzein derivatives could form non-covalent complexes with the protein lysozyme, while non-covalent complexes were not detected in the mixed solution of daidzein with lysozyme. Relative affinity of every non-covalent complex was obtained according to its different decomposition orifice voltage.Daidzein （7,4＇-dihydroxyisoflavone） was phosphorylated by a modified Atherton-Todd reaction. The structures of the five target product, were determined by X-ray, IR, NMR and ESI-MS. Electrospray ionization results show that in the gas phase all the phosphorylated daidzein derivatives could form non-covalent complexes with the protein lysozyme, while non-covalent complexes were not detected in the mixed solution of daidzein with lysozyme. Relative affinity of every non-covalent complex was obtained according to its different decomposition orifice voltage.

关 键 词：DAIDZEIN PHOSPHORYLATION ESI-MS non-covalent complex 

分 类 号：O641.4[理学—物理化学]