低温下钙对‘Page’橘柚愈伤组织抗寒相关生理指标的影响  

Effect of Calcium on Physiological Indexes of ‘Page’ Tangelo Callus Under Low Temperature

作　　者：李新国[1] 张建霞[1] 孙中海[1] 

机构地区：[1]华中农业大学国家柑橘育种中心园艺植物生物学教育部重点实验室,武汉430070

出　　处：《应用与环境生物学报》2007年第3期322-326,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金资助项目(No.30070528)~~

摘　　要：研究了低温下外源不同Ca2+浓度对‘Page’橘柚愈伤组织一些抗寒相关的生理生化指标的影响.结果表明,以5mmolL-1的低浓度Ca2+处理比缺钙处理显著降低了低温下‘Page’橘柚愈伤组织细胞膜的伤害率和膜脂过氧化产物MDA的积累,显著提高了可溶性蛋白质的含量以及SOD和POD的活性,因而增强了‘Page’橘柚愈伤组织的抗寒性.但以15mmolL-1的高浓度Ca2+处理时,表现出一定的“毒害”作用,反而降低了抗寒性.以上结果表明,一定浓度范围内的外源Ca2+与‘Page’橘柚抗寒性的提高程度密切相关.Effect of different concentrations of extraneous Ca^2＋ on some physiological and biochemical indexes of ‘ Page＇ tangelo callus was investigated at 3 ℃. The results indicated that the treatment with 5 mmol L^- 1 of extraneous Ca^2＋ could significantly reduce membrane injury rate and decrease malondialdehyde （MDA） accumulation, and it could significantly increase the content of soluble protein and enhance the activities of superoxide dismutase （SOD） and peroxidase （POD）. As a result, it strengthened cold resistance of ‘ Page＇ tangelo callus, although 15 mmol L^-1 Ca^2＋ played a negative role. Consequently, the cold hardiness of ‘ Page＇ tangelo callus was closely related to the concentrations of extraneous Ca^2＋. Tab 5, Ref 28

关 键 词：Ca2^+ 'Page’橘柚愈伤组织 膜伤害率 MDA SOD POD 可溶性蛋白质 

分 类 号：S666[农业科学—果树学]