肌醇六磷酸对人结肠细胞系HT-29细胞周期的影响  

EFFECTS OF INOSITOL HEXAPHOSPHATE (IP_6) ON CELL CYCLE OF HT-29 HUMAN COLON CARCINOMA CELL LINE

作　　者：宋扬[1] 张铮[1] 马葆兰 

机构地区：[1]青岛大学医学院医学营养研究所,青岛266021 [2]中国人民解放军401医院,青岛266071

出　　处：《营养学报》2007年第3期246-249,共4页Acta Nutrimenta Sinica

摘　　要：目的:研究肌醇六磷酸(IP6)对HT-29细胞周期的影响并探讨其作用机制。方法:应用四甲基偶氮唑蓝实验(MTT法)观察IP6对HT-29细胞增殖的影响;流式细胞仪检测不同浓度IP6作用72h对HT-29细胞周期的影响;应用免疫细胞化学法检测IP6对HT-29细胞内突变型p53、细胞周期抑制蛋白p21表达的影响。结果:IP6对结肠癌细胞株的生长具有抑制作用,且具有浓度依赖和时间依赖关系。经IP6作用处理的HT-29细胞的细胞周期发生G1期阻滞。与对照组比,各IP6浓度组均能抑制突变型p53蛋白的表达(P<0.05),上调p21蛋白的表达(P<0.05)。结论:IP6对HT-29细胞的生长具有明显的抑制作用。其机制可能是IP6降低p53蛋白的异常表达,刺激p21蛋白的表达,使细胞周期发生G1期阻滞,从而起到抑制细胞增殖的作用。Objective： To investigate the effects and mechanisms of inositol hexaphosphate （IP6） on cell cycle of HT-29 human colon carcinoma cell line. Method： HT-29 cells were exposed to various concentrations of IP6 for certain time. The effect of IP6 on cells proliferation was evaluated by MTT assay. Flow cytometric analysis was performed for cell cycle progression. Immunocytochemical stain was used to detect the expression of p53 and p21 protein. Results： A significant dose-dependent as well as time-dependent growth inhibition was observed in IP6-treated HT-29 cells, and associated with an increase in G1 arrest; Different concentrations of IP6 decreased the abnormal expressions of p53 gene and strongly increased the expression of p21. Conclusions： IP6 had an inhibitory effect on proliferation of HT-29 cells .and its mechanisms might be related to many factors, such as inhibiting the abnormal expression of p53 gene, inducing the expression of cell cycle inhibitor p21 which block the cell cycle.

关 键 词：肌醇六磷酸 结肠癌 HT-29细胞系 细胞周期 

分 类 号：R735.3[医药卫生—肿瘤]