肿瘤患者外周血单个核细胞来源DC的体外诱导及其功能  

Dendritic cells derived from peripheral blood monocytes of patients with malignant tumor:a study of in vitro activity and function

作　　者：朱一蓓[1] 张学光[1] 黄勇[1] 陈成[1] 李文香[1] 戴俊[1] 夏瑜[1] 吴明媛[1] 

机构地区：[1]苏州大学医学生物技术研究所

出　　处：《中国肿瘤生物治疗杂志》2007年第3期264-268,共5页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金重点项目(No30330540)~~

摘　　要：目的:研究肿瘤患者外周血单个核细胞来源的树突状细胞(monocyte-deriveddendriticcells,Mo-DC)共刺激分子的表达及其功能。方法:选取11例肺癌、卵巢癌和胃癌患者及10例正常志愿者,分别经粒细胞巨噬细胞集落刺激因子(gran-ulocytemacrophagecolonystimulatingfactor,GM-CSF)和白细胞介素4(IL-4)诱导外周血单个核细胞来源的DC,通过免疫荧光标记和流式细胞术分析、混合淋巴细胞反应(MLR)、FITC-dextran吞噬实验、微孔迁徙实验以及酶联免疫吸附试验(ELISA)等方法比较肿瘤患者和健康志愿者外周血单个核细胞来源DC的表型及功能差异。结果:肿瘤患者来源的Mo-DC在体外诱导早期持续高表达编程死亡-1配体1(programmeddeath-1ligand1,PD-L1),而PD-L2及CCR7、CXCR4的表达则较正常人低(P<0.05);患者来源的Mo-DC分泌高水平的IL-10,体外激发自体T细胞增殖及趋化T细胞尤其是趋化活化T细胞的能力明显低于正常人(P<0.05)。结论:肿瘤患者来源的Mo-DC可能由于自分泌高水平的IL-10和高表达PD-L1而影响其功能的行使。Objective： To investigate the expression of costimulatory molecules of dendritic cells derived from monocytes obtained from the peripheral blood of patients with malignant tumor and their functions. Methods： Peripheral blood samples were obtained from I 1 patients with lung cancer, ovarian cancer, or gastric cancer and 10 normal volunteers; the monocyte-derived dendritic cells were generated in vitro using GM-CSF and IL-4. The phenotypes and functions of DCs derived from healthy volunteers and cancer patients were compared using immunofluorescence, FCT, mixed-lymphocyte reaction, FITC-dextran capture, ELISA, and chemotaxis activity of T lymphocytes. Results： DCs derived from cancer patients expressed higher level of PD-L1 at the early stage of the in vitro culture, but lower levels of PD-L2, CCR7 and CXCR4 compared with those derived from healthy volunteers. DCs derived from cancer patients secreted high level of IL-10 and had a retarded ability to stimulate T cells proliferation and to enrich T cells, especially activated T cells, compared with DCs derived from healthy volunteers. Conclusion： High expression of PD-L1 and autocrine of IL-10 may impair the function of DCs derived from cancer patients.

关 键 词：树突状细胞 编程死亡-1配体1 白细胞介素10 肿瘤免疫 

分 类 号：R730.2[医药卫生—肿瘤]