PLGA为佐剂的OVA纳米疫苗皮下注射可预防小鼠过敏反应性气道炎症和调节Th1/Th2反应  

Subcutaneous injection of PLGA-encapsulated OVA nanoparticles reduces the respiratory inflammation and modifies Th2/Th1 reaction in a mouse asthma model

作　　者：喻海琼[1] 刘志刚[1] 曾琼[1] 

机构地区：[1]深圳大学过敏反应与免疫学研究所

出　　处：《免疫学杂志》2007年第4期366-369,共4页Immunological Journal

基　　金：国家"863"计划(2002AA214011);国家自然科学基金(30271226;30471505);粤港关键领域重点突破项目(20054982207);广东省科技计划重大项目(2003C104019);深圳市科技计划(200218)资助

摘　　要：目的探讨聚乳酸-羟基乙酸共聚物[poly(D,L-lactic-co-glycolic)acid,PLGA]包裹的卵清蛋白(OVA)纳米疫苗(POM)对哮喘小鼠的免疫治疗效果。方法包裹不同剂量(低、中、高)的OVA纳米粒子和对照(OVA、空白纳米粒子、PBS)通过皮下注射给予小鼠,再用OVA进行致敏和激发,通过肺组织学、支气管肺泡灌洗液(BALF)细胞计数、测定BALF和脾细胞培养上清液中细胞因子的含量,观察小鼠呼吸道炎症和免疫学改变。结果肺部组织学和BALF中细胞计数结果显示,与PBS对照组相比,OVA治疗组、中剂量和高剂量OVA纳米组的肺部嗜酸性浸润显著减轻,BALF中总细胞和嗜酸性细胞显著减少。细胞因子测定结果显示,与PBS对照组相比,中、高剂量OVA纳米组的BALF和脾细胞培养上清液中IFN-γ显著升高,IL-4水平显著降低。OVA治疗组中IL-4水平显著下降,而IFN-γ水平无显著差异。结论OVA纳米疫苗可预防哮喘嗜酸性气道炎症,其可能的机制之一是调节了过敏性哮喘的Th1/Th2失平衡反应。Objective To investigate the potential effects of PIGA [ poly （D, L-lactic-co-glycolic ） acid ] encapsulated OVA nanoparticles （POM） on mouse asthma model. Methods PLGA-encapsulated OVA nanoparticles with various doses （low, mid, and high） and control fluids （OVA solution, empty nanoparticles, and PBS） were subcutaneously administered to mice respectively, and then the mice were primed and challenged with OVA. The respiratory inflammational and immunological reactions were demonstrated by the lung histology, the eosinophils in bronchoalveolar lavage fluid （BALF）, and the cytokine levels in BALF and spleen culture supernatants. Results The eosinophilic infiltration in lungs was greatly reduced in high or mid-dose-POM treatment groups and OVA treatment group than that of PBS control. Cytokine profiles revealed higher IFN-γ and lower IL-4 levels in BALF and splenocyte culture supernatants of high or mid-dese-POM treatment groups in comparisov with PBS control group. The concentration of IL-4 in OVA treatment group was significantly lower than that of PBS control, while IFN-γ level had no significant changes comparing with that of PBS control. Conclusion The POM had preventive effects against asthmatic inflammation, and may modulate the unbalanced Th2 and Th1 reaction in allergic diseases.

关 键 词：哮喘 OVA PIGA Th1/Th2反应 

分 类 号：R392.8[医药卫生—免疫学]