重组人MUC1-MBP融合蛋白诱导小鼠Th1活化  

Recombinant MUC1-MBP fusion protein activates mouse Th1 cells

作　　者：张淑芳[1] 台桂香[1] 马吉春[1] 高航[1] 

机构地区：[1]吉林大学基础医学院免疫学教研室,长春130021

出　　处：《免疫学杂志》2007年第4期373-375,共3页Immunological Journal

基　　金：教育部留学归国启动基金资助课题(9922G167)

摘　　要：目的检测重组MUC1-MBP融合蛋白对小鼠Th1细胞的活化作用。方法通过生物活性法测定MUC1-MBP免疫鼠脾细胞培养上清液中IL-2I、FN和血清中TNF水平;通过免疫组织化学染色法检测CD4+T细胞在肿瘤组织中的浸润。结果MUC1-MBP免疫鼠脾细胞分泌IL-2和IFN及血清TNF水平较对照组明显增高;MUC1-MBP诱导小鼠CD4+T细胞在肿瘤组织中浸润。结论重组人MUC1-MBP融合蛋白可激活小鼠Th1细胞。Objective To investigate the activity of Thl cells in mice immunized with recombinant MUC1-MBP fusion protein. Methods The levels of IL-2 and IFN secreted by splenocytes and the serum TNF were detected in mice immunized by recombinant MUC1- MBP. Immunohistechemistry technique was used to analyze the infdtrafion of CD4^＋ T cells into cancerous tissue of MUC1-MBP immunized mice. Results The levels of IL-2 and IFN secreted by splenocytes and the serum TNF were higher in MUC1-MBP-immunized mice than those in the groups of negative control. MUC1-MBP induced the infiltration of CD4^＋ T cells into cancerous tissue. Conchtsion The recombinant MUC1-MBP fusion protein can activate Thl cells of mouse.,

关 键 词：MUC1-MBP融合蛋白 TH1细胞 细胞因子 

分 类 号：R73-36[医药卫生—肿瘤]