氧化应激对猪眼小梁细胞内皮细胞白细胞黏附分子-1表达的影响  

Effects of Oxidative Stress on the Expression of Endothelial-leukocyte Adhesion Molecule-1 in Porcine Trabecular Meshwork Cells

作　　者：周崎[1] 刘玉琴[2] 赵家良[1] 张华[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院眼科,北京100730 [2]中国医学科学院北京协和医学院基础医学研究所细胞中心,北京100005

出　　处：《中国医学科学院学报》2007年第3期394-397,I0008,共5页Acta Academiae Medicinae Sinicae

基　　金：高等学校博士学科点专项科研基金(20030023058)~~

摘　　要：目的研究氧化应激对猪眼小梁细胞内皮细胞白细胞黏附分子-1(ELAM-1)表达的影响,探讨氧化应激诱导的ELAM-1的表达与白细胞介素1α(IL-1α)的关系。方法原代培养的猪眼小梁细胞经过血清饥饿培养后,用不同浓度白细胞介素-1受体拮抗剂(IL-1rα)处理或直接用1mmol/L的H2O2刺激后,用免疫细胞化学法检测小梁细胞E-LAM-1的表达。结果H2O2处理的原代培养的猪眼小梁细胞中ELAM-1表达呈阳性,高浓度(180μg/ml和600μg/ml)拮抗剂IL-1rα预处理的猪眼小梁细胞ELAM-1表达呈阴性。结论氧化应激可诱导猪眼小梁细胞表达ELAM-1。在体外细胞培养体系,高浓度的IL-1rα可拮抗氧化应激对ELAM-1表达的作用。Objective To investigate the effects of oxidative stress on the expression of endothelialleukocyte adhesion molecule-1 （ ELAM-1 ） in porcine trabecular meshwork cells and to elucidate the relationship between the expression of ELAM-1 and IL-1 ot. Methods Primary cultured porcine trabecular meshwork cells were cultured without serum and treated with 1 mmol/L H2O2 with or without pretreatment of IL-1 rot of different concentrations. The expression of ELAM-1 was tested by immunocytochemistry. Results ELAM-1 immunocytochemistry staining was positive in the H2O2 -treated porcine trabecular meshwork cells. It was negative in the porcine trabecular meshwork cells pretreated with IL-1 rot of high concentrations （ 180μg/ml and 600μg/ml）. Conclusions Oxidative stress can induce the expression of ELAM-1 in porcine trabecular meshwork cells. In the in vitro cell system, IL-1 rot of high concentration can inhibit the expression of ELAM-1 induced by oxidative stress.

关 键 词：氧化应激 小梁细胞 内皮细胞白细胞黏附分子-1 

分 类 号：R775[医药卫生—眼科] R392.11[医药卫生—临床医学]