基于DNA barcoding(条形码)技术的中药材鉴定  

Use of DNA barcoding to identify Chinese medicinal materials

作　　者：陈士林[1] 姚辉[1] 宋经元[1] 李西文[1] 刘昶[2] 陆建伟 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094 [2]香港大学李嘉诚医学院分子中药实验室 [3]国家中医药管理局科技司,北京100026

出　　处：《世界科学技术-中医药现代化》2007年第3期7-12,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金项目(30572324):濒危品种川贝母光合蒸腾特性与野生抚育生态因子的相关性研究

摘　　要：DNA条形码(DNA barcoding)是根据对一段标准的DNA序列的分析来鉴定物种,已成为生物物种鉴定的新方向,受到世界40多个国家130多个组织中传统生物分类学家、分子生物学家和生物信息学家等多学科专家的关注。本文通过介绍DNA条形码的产生、发展和研究现状,探讨其在中药材鉴定中应用的技术方法、技术路线、关键问题以及应用范围,并展望了DNA条形码在中药材鉴定中的应用前景。To identify biological species, DNA barcoding as a new technique is developed. DNA barcoding sequences from a standardized and agreed -upon position in the genome are very short relative to the entire genome and they can be obtained reasonably quickly and cheaply, which attracted more than 130 organizations from 40 countries in the globe. This paper reviews the appearance and development of DNA barcoding. Simultaneously, we study the methods, stepwise technique map and key questions of DNA barcoding which was used to identify Chinese medicinal materials. In conclusion, DNA barcoding has a good prospect in authentication of Chinese medicinal materials.

关 键 词：中药材 DNA条形码 鉴定 

分 类 号：R394.8[医药卫生—医学遗传学]