Preparation and Characterization of Three Monoclonal Antibodies against HIV-1 p24 Capsid Protein  

作　　者：Guangjie Liu Jianping Wang Jianchun Xiao Zhiwei Zhao Yongtang Zheng 

机构地区：[1]Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China [2]Key Laboratory of Conservation and Utilization for Bio-resources, Yunnan University, Kunming, Yunnan 650091, China

出　　处：《Cellular & Molecular Immunology》2007年第3期203-208,共6页中国免疫学杂志（英文版）

基　　金：grants from the National Natural Science Foundation of China （39500137, 30471605, 30671960）; the Natural Science Foundation of Yunnan （95C0099Q）; Key Technological R＆D Program of China （2004BA719A14） and Yunnan （2004NG12）; CAS Projects （KSZ85-0108, STZ-01-17; KSCX2-SW-216; KSCX 1-SW- 11, KSCX 1-YW-R- 15）.

摘　　要：HIV-1 p24 detection provides a means to aid the early diagnosis of HIV-1 infection, track the progression of disease and assess the efficacy of antiretroviral therapy. In the present study, three monoclonal antibodies （mAbs） p3JB9, p5F1 and p6F4 against HIV-1 p24 were generated. All mAbs could detect p24 of HIV-1ⅢB, HIV-1Ada-M, HIV-174v mAbs p5F1 and p6F4 could detect HIV-1KM018, while p3JB9 could not. Three mAbs did not react with HIV-2ROD, HIV-2CBL-20 and SIVagmTYO-1. The recognized epitope of p5F1 was located on the Gag amino acid region DCKTILKALGPAATLEEMMTAC. The p5F1 was used to establish a modified sandwich ELISA with rabbit anti-p24 serum and showed good specificity and high sensitivity, which has been used to measure HIV-1 p24 antigen levels in research. Cellular ＆ Molecular Immunology.HIV-1 p24 detection provides a means to aid the early diagnosis of HIV-1 infection, track the progression of disease and assess the efficacy of antiretroviral therapy. In the present study, three monoclonal antibodies （mAbs） p3JB9, p5F1 and p6F4 against HIV-1 p24 were generated. All mAbs could detect p24 of HIV-1ⅢB, HIV-1Ada-M, HIV-174v mAbs p5F1 and p6F4 could detect HIV-1KM018, while p3JB9 could not. Three mAbs did not react with HIV-2ROD, HIV-2CBL-20 and SIVagmTYO-1. The recognized epitope of p5F1 was located on the Gag amino acid region DCKTILKALGPAATLEEMMTAC. The p5F1 was used to establish a modified sandwich ELISA with rabbit anti-p24 serum and showed good specificity and high sensitivity, which has been used to measure HIV-1 p24 antigen levels in research. Cellular ＆ Molecular Immunology.

关 键 词：monoclonal antibody HIV-1 P24 EPITOPE ELISA 

分 类 号：R512.91[医药卫生—内科学]