TLR4(896A>G)基因错义突变与重型急性胰腺炎胰腺坏死组织感染的相关性研究  

Association Between Toll Like Receptor 4(896A>G)Mutations and Pancreatic Necrotic Infection in Severe Acute Pancreatitis

作　　者：高宏凯[1] 张新国[1] 周总光[2] 李园[2] 陈友岱[2] 

机构地区：[1]武警总医院普外科,北京100039 [2]四川大学华西医院普外科,成都610041

出　　处：《四川大学学报（医学版）》2007年第4期617-619,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家杰出青年科学基金(批准号39925032)资助

摘　　要：目的研究Toll样受体4(TLR4)896A>G基因错义突变与重型急性胰腺炎(SAP)胰腺坏死组织感染的相关性。方法采用错配PCR-限制性片段长度多态性(RFLP)分析方法在SAP伴胰腺组织坏死的病人和健康自愿者人群中检测TLR4(896A>G)的等位基因频率。结果1所有TLR4(896A>G)的基因突变均为杂合子;2SAP胰腺组织坏死伴胰腺感染组(50例)TLR4基因错义突变频率为20%,与健康志愿组TLR4基因错义突变频率5.6%相比,TLR4基因错义突变频率明显升高,差异有统计学意义(P=0.019);3在SAP伴感染病人中,TLR4基因错义突变组G-细菌感染率为44.1%(15/34),明显高于无TLR4基因错义突变组18.5%(15/81),差异有统计学意义(P=0.04)。结论TLR4(896A>G)基因错义突变与SAP胰腺坏死组织的感染相关联。Objective To determine the relationship between Toll like receptor （TLR） 4 （896A 〉G） mutations and pancreatic necrotic infection in acute pancreatitis （AP）. Methods TLR4 （896A〉G） mutations were detected by mispairing PCR-RFLP analysis technique in the patients with pancreatic necrosis and healthy volunteers. Results ① All of the TLR4 mutations were heterozygotes; ②The G allele frequencies of TLR4 genes were significantly higher in the patients with pancreatic infection than in the healthy volunteers; ③The incidence of gram-negative infection was significantly higher in the patients with the TLR4 mutations [15（44.1%） of 34] than that in the wild type population [15（18.5%） of 81]. Conclusion TLR4 （896A〉G） mutations are associated with the infection of pancreatic necrosis in the patients with AP.

关 键 词：TOLL样受体4 基因突变 重型急性胰腺炎 胰腺坏死 感染 

分 类 号：R657.51[医药卫生—外科学]