小鼠腹腔内腺病毒介导的基因表达和分布  

GENE EXPRESSION AND DISTRIBUTION IN MOUSE ABDOMINAL CAVITY MEDIATED BY ADENOVIRUS

作　　者：吴昊泉[1] 耿雨红[1] 徐人尔[1] 应蓓蓓[1] 孙加廷 王义斌 赵寿元[1] 李昌本[1] 

机构地区：[1]复旦大学遗传学研究所,遗传工程国家重点实验室 [2]复旦大学立人实验室 [3]Scripps Research Institute,La Jolla,CA 92037,USA

出　　处：《实验生物学报》1997年第1期65-71,共7页Acta Biologiae Experimentalis Sinica

摘　　要：本文报道了重组腺病毒——带有报道基因1acZ的Ad/RSV-β-gal注入小鼠腹腔后,在胃、小肠、大肠、子宫、肝、脾、膀胱、体壁、膈和睾丸的腹膜面均出现被X-gal染成蓝色的细胞,腹膜面下未见染色细胞。结果表明重组腺病毒可广泛感染腹腔内的组织并使外源基因表达,但不扩散出体腔。另一带有单钝疱疹病毒胸苷激酶基因(HSV-tk)的重组腺病毒Ad/RSV- tk,注入小鼠腹腔后并给以药物ACV,未发现急性毒性反应。在此基础上,讨论了TK/ACV 系统对腹腔内肿瘤作基因治疗的可行性。Infection and expression of recom-binant human adenovirus in mouse abdominal cavity was reported. After adenovirus vector Ad/RSV-β-gal harboring the E. coli lacZ marker gene was injected into mice abdominal cavity, the peritoneal surface of jejunum, ileum, colon, uterus, liver, spleen, stomach, bladder, abdominal wall, diaphragm and testis was found large patches of lacZ-positive cells. But the adenovirus vector was not able to pene- trate the peritoneum, as demonstrated by histochemical staining. Another adenovirus vector Ad/RSV-tk harboring the HSV-tk gene was injected into mouse abdominal cavity and the mouse was treated with ACV. No acute toxic reaction was observed. Based on these data, the feasibility of gene therapy of malignant tumor within abdominal cavity with adenovirus mediated TK/GCV system was discussed.

关 键 词：基因表达 腺疾病 载体 基因治疗 

分 类 号：Q753[生物学—分子生物学] R394[医药卫生—医学遗传学]