羟基脲(Hydroxyurea)对细胞周期与珠蛋白基因表达的影响(简报)  

THE EFFECTS OF HYDROXYUREAON CELL-CYCLE DISTRIBUTION AND THE EXPRESSION OF HUMAN β-GLOBIN GENE IN K 562 CELLS

作　　者：蒋俶 戴长虹 谢恒月[1] 陈雅娣 胡炜 龚钟萍 钱若兰[1] 

机构地区：[1]中国科学院上海细胞生物学研究所,上海200031

出　　处：《实验生物学报》1997年第1期109-114,共6页Acta Biologiae Experimentalis Sinica

基　　金：中国科学院生命科学联合开放实验室;国家自然科学基金

摘　　要：已有许多证据表明羟基脲能增加镰状细胞贫血及β-地贫病人的胎儿型血红蛋白(HbF)的合成。最近,有人报道羟基脲也能使一些患有β-地贫病人的β-珠蛋白基因表达增加。K562细胞是人红白血病细胞株,它只能表达胚胎型(ε-)与胎儿型(γ-)珠蛋白基因,而不能表达成年型(β-)珠蛋白基因。因此。Although the hydroxyurea (HU) has been extensively studied, little is known of its molecular mechanism in controlling the expression of human globin gene and in modulating the progression of cell-cycle in K 562 cell. In the present study, the effect of hydroxyurea on proliferative kinetics of K 562 cells was examined by monitoring the number of cells during a period of 8 day's cell culture. Our results showed that there was a dose related decrease in cell growth when K562 cells were incubated with HU. Moreover, cell-cycle analysis demonstrated that HU had profound effect on cell-cycle distribution. In the case of the induced K 562 cells, there was an increased accumulation of cells in S phase and a decreased fraction of cells in G 1 and G 2 + M phase. Furthermore, HU could induce the expression of human β-glo-bin gene in the induced K 562 cells. Our results indicate that HU has a potential to inhibit the proliferation of K 562 cells and to stimulate the terminal differentiation of this cell.

关 键 词：细胞周期 珠蛋白基因 羟基脲 肿瘤 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R730.21[医药卫生—基础医学]