氟哌啶醇和R(-)-NPA抑制C_6神经胶质瘤细胞的钾电流  

INHIBITION OF VOLTAGE-DEPENDENT K^+ CURRENTS IN RAT C_6 GLIOMA CELLS BY HALOPERIDOL AND R(-)-NPA

作　　者：谢重[1] 何淑舫[1] 

机构地区：[1]中国科学院上海脑研究所,上海200031

出　　处：《生理学报》1997年第1期45-53,共9页Acta Physiologica Sinica

基　　金：上海生命科学联合开放研究实验室资助!932105

摘　　要：本工作用全细胞膜片箝记录的方法，观察了氟哌啶醇（haloperidol，以下简称HALO）和R（-）-Propyinoramorphine（以下简称NPA）对C6神经胶质瘤细胞（C6gliomacells）的电压依赖性钾电流的作用，并初步分析了它们的作用机制。结果表明，HALO和NPA都能抑制C6细胞的K+流，其抑制作用是可逆的，而且都与药物浓度有依赖关系。两者所抑制的主要是K+电流中的慢成分，而对快成分的作用有所不同。它们的抑制作用不是由多巴胺D2受体介导的，也不是通过G-蛋白或细胞内钙作用的，很可能是直接作用于钾通道。In this study, the inhibitory effects of R (-) - NPA (R (-) - Propylnorapomorphine) and haloperidol on voltage-dependent K+ currents in rat C6 glioma cells (C6 eells) were observed by means of the whole-cell patch-clamp recording technique.The mechanisms of the effects were aiso analyzed. The main results were as follows:(1) Both R (-) -NPA and haloperidol could inhibit K+ currents dose-dependently andreversibly in C6 cells. (2) R (-) -NPA and haloperidol inhibited mainly the slow component of the K+ currents. (3) These inhibitory respollas were not mediated bydopamine receptors or dependent on G-proteins and intracellular calcium. It was suggested they refer to some direct action on K+ channels.

关 键 词：神经胶质瘤 癌细胞 氟哌啶醇 多巴胺受体 钾电流 

分 类 号：R730.264[医药卫生—肿瘤] R73－34[医药卫生—临床医学]