血浆EB病毒DNA定量测定对鼻咽癌的诊断价值  

The diagnostic value of quantitative analysis of plasma Epstein-Barr virus DNA in patients with nasopharyngeal carcinoma

作　　者：廖勇[1] 罗志强[1] 

机构地区：[1]衡阳市南华大学附属第二医院耳鼻咽喉科,湖南衡阳421001

出　　处：《中国耳鼻咽喉颅底外科杂志》2007年第3期186-188,共3页Chinese Journal of Otorhinolaryngology-skull Base Surgery

摘　　要：目的分析血浆EB病毒(Epstein-Barr virus,EBV)DNA定量测定对鼻咽癌的诊断价值。方法采用荧光定量聚合酶链反应(fluorescence quantitative polymerase chain reaction,FQ-PCR)技术,测定35例经病理确诊的鼻咽癌患者血浆EB病毒DNA含量,并与50例健康对照者比较。结果鼻咽癌患者血浆EBV-DNA阳性率和复制量均高于健康对照者。两组阳性率和中位浓度的比较均有统计学差异。结论血浆EBV-DNA定量分析不能作为临床上确诊鼻咽癌的方法,但对鼻咽癌的筛查有实用价值,值得进一步研究。Objective To elucidate the diagnostic value of quantitative analysis of plasma Epstein-Barr virus deoxyribonucleic acid （EBV-DNA） in patients with nasopharyngeal carcinoma （NPC）. Methods The copy of plasma EBV-DNA was detected with fluorescence quantitative polymerase chain reaction（FQ-PCR）method in 35 samples of NPC patients, and 50 healthy adults were taken as normal control Results The positive rate and copy of plasma EBV-DNA of NPC patients were significantly higher than those of normal controls. Conclusion FQ-PCR determination of plasma EBV -DNA can not be used for confirming the diagnosis of NPC, but is of practical use to screen NPC. Further investigation is deserved.

关 键 词：鼻咽肿瘤 荧光定量PCR 血浆 EBV-DNA 

分 类 号：R739.62[医药卫生—肿瘤]