皮脂腺癌与汗腺癌细胞p53蛋白的定量分析  

Quantitative analysis of p53 protein in sebaceous gland carcinoma and sweat gland carcinoma

作　　者：四荣联[1] 李玉平[1] 姚春华[1] 张贵贞[1] 孙修蓉[1] 

机构地区：[1]河北医科大学第二医院皮肤科

出　　处：《临床皮肤科杂志》1997年第1期3-5,共3页Journal of Clinical Dermatology

摘　　要：应用流式细胞免疫技术，对１８例皮脂腺癌及汗腺癌患者细胞的ｐ５３蛋白进行了定量分析。结果表明：癌细胞与正常对照细胞的增殖指数、ＤＮＡ指数及ｐ５３蛋白相对含量之间有显著的差别。癌细胞的异倍体率及ｐ５３蛋白的阳性表达率分别为７２．２２％和１００％，对照细胞的ｐ５３蛋白均为阴性表达。由此可见皮脂腺癌和汗腺癌细胞具有很高的增殖活性及ｐ５３基因的过度表达。The p53 protein in eighteen cases of sebaceous gland carcinoma and sweat gland carcinoma was analyzed quantitatively with flow cytometry and immunofluorescence staining technique. The results showed that there were significant differences in DI(DNA Index), PI(Proliferating Index) and FI(Fluorescence Index) of p53 protein between cancer cells and normal cells. The rates of heteroploid and positive expression of p53 protein in cancer cells were 72.22% and 100% respectively, while the normal cells showed negative expression. The results suggest that the cancer cells in sebaceous gland carcinoma and sweat gland carcinoma possess the characteristic of highly proliferative activity and over expression of p53 gene product.

关 键 词：皮脂腺癌 汗腺癌 皮肤肿瘤 P53蛋白 定量分析 

分 类 号：R739.5[医药卫生—肿瘤]