实验性大鼠肝纤维化一氧化氮动态变化及意义  

IMPLICATION OF DYNAMIC CHANGES OF NITRIC OXIDE IN RATS OF EXPERIMENTAL LIVER FIBROSIS

作　　者：方建珍[1] 高洁生[1] 吴轰[1] 杨锡兰[1] 刘跃飞[1] 

机构地区：[1]湖南医科大学附属第二医院中心实验室

出　　处：《免疫学杂志》1997年第1期26-28,共3页Immunological Journal

基　　金：国家自然科学基金

摘　　要：用ＣＣＬ４复制肝纤维化大鼠模型，于第４、８、１２ｗｋ分别采血，取腹腔巨噬细胞和肝组织，测定血中ＮＯ、ＴＮＦα、ｓＩＬ－２Ｒ和腹腔巨噬细胞培养上清中ＮＯ、ＴＮＦα，同时作肝脏组织病理检验。结果显示：随着肝纤维化的进展，血清ＮＯ、ＴＮＦα、ｓＩＬ－２Ｒ的含量递增，约在第８ｗｋ达到高峰，ＮＯ的增高较上述其他指标更明显，呈正相关（ｒ＝０．８８０５）。肝纤维化与腹腔巨噬细胞产生的ＮＯ、ＴＮＦα的量呈正相关（ｒ＝０．８５６５）。提示ＮＯ参与了肝损伤。ＮＯ也是反应肝纤维化的敏感指标。In order to investigate the implication of dynamic changes of nitric oxide(NO)in rats of experimental liver fibrosis.The rat models of liver fibrosis were induced by CCL 4,NO,TNFα,sIL 2R in serum and cultured peritoneal macrophages were measured at 4,8 and 12 week.Histological changes were analyed by pathalogical method.As the liver fibrosis was developed,the NO,TNFα and sIL 2R were gradually increasing,and got the highest point at eighth week.Especially,the NO level in serum was significantly increased and positively related with the degree of liver fibrosis( r =0.8805).The results suggest that NO could induce the damage of liver cells.Meanwhile,NO level in serum also a sensitive indicator for showing liver fibrosis.

关 键 词：一氧化氮 肝纤维化 动物模型 

分 类 号：R575.2[医药卫生—消化系统]