人脑星形细胞肿瘤IL-8的表达及其与血管生成的关系  

Expression of IL-8 in astrocytomas and relationship with angiogenesis

作　　者：吴娜[1] 王清良[1] 卞修武[1] 

机构地区：[1]第三军医大学附属西南医院病理学研究所,重庆400038

出　　处：《国际病理科学与临床杂志》2007年第3期198-201,共4页Journal of International Pathology and Clinical Medicine

基　　金：国家自然科学基金(30370552)~~

摘　　要：目的:探讨IL-8,VEGF在脑胶质瘤中的表达及与血管生成的关系。方法:应用由45例人脑星形细胞肿瘤和6例正常脑组织组成的组织芯片,采用免疫组织化学技术分别进行IL-8,VEGF,CD34标记并进行半定量,观测在不同病理分级胶质瘤中的表达及与微血管密度(MVD)之间的关系。结果:6例正常脑组织中不表达IL-8,VEGF。45例脑胶质瘤中,27例IL-8呈阳性表达,32例VEGF呈阳性表达,Ⅲ,Ⅳ级脑胶质瘤中IL-8,VEGF的表达比Ⅰ级、Ⅱ级明显增强,IL-8,VEGF评分为强阳性的胶质瘤内MVD明显高于评分为阴性和阳性的MVD(P<0.01)。IL-8表达与MVD呈正相关(rs=0.64,P<0.01)。VEGF表达与MVD之间呈正相关(rs=0.44,P<0.01)。IL-8表达与VEGF表达之间亦呈正相关(rs=0.56,P<0.01)。结论:IL-8,VEGF的表达与胶质瘤病理分级、MVD密切相关,二者在胶质瘤的血管生成中可能相互关联、共同调节肿瘤血管生成。Objective To explore the relationship between interleukin-8 （IL-8） and vascular endothelial growth factor （VEGF） expression and angiogenesis in human gliomas. Methods Immunohisto- chemical staining was performed on the tissue array constructed with 45 cases of human astrocytomas and 6 cases of normal brains to detect expressions of IL-8, VEGF and CD34, and observe the correlation be- tween IL-8 and VEGF expression levels and microvessel density （MVD） in astrocytomas with different pathologic grades. Results IL-8, VEGF in 6 cases of normal brain were undetectable. In 45 cases of gliomas, 27 cases were IL-8 positive, 32 cases VEGF positive. The MVD were significantly higher in IL- 8 and VEGF positive cases than in mild positive or negative cases. The IL-8 and VEGF expressions were correlated with MVD. IL-8 expression was positively correlated with VEGF expression. Conclusion The expressions of IL-8 and VEGF have a correlation with histologic grade and MVD of astrocytoma, and both regulate the tumor angiogenesis in gliomas.

关 键 词：脑胶质瘤 白细胞介素-8 血管内皮生长因子 血管生成 组织芯片 

分 类 号：R739.4[医药卫生—肿瘤]