SPF级BALB/c小鼠感染幽门螺杆菌的造模方法研究  

Research of Methods of SPF grade BALB/c Mice Model with H.pylori Infection

作　　者：刘翔[1] 卢放根[1] 

机构地区：[1]中南大学湘雅二医院消化内科,湖南长沙410011

出　　处：《临床和实验医学杂志》2007年第7期3-5,共3页Journal of Clinical and Experimental Medicine

摘　　要：目的比较现有的幽门螺杆菌(Hp)菌液直接灌胃造模法和预先用抗生素灌胃破坏原籍菌群再用Hp灌胃造模法效果的差异,以建立一种造模周期短且可靠的Hp感染的慢性胃炎模型。方法60只SPF级BALB/c小鼠随机分为三组,抗生素造模组用抗生素灌胃后再予Hp菌悬液灌胃造模,直接造模组予Hp菌悬液灌胃造模,生理盐水对照组予生理盐水灌胃对照。小鼠均于灌胃后8周处死。用快速尿素酶试验、Giemsa染色和微需氧细菌培养检测Hp定植,用选择性培养基培养法分析造模前后小鼠胃内菌群的变化,用H-E染色检查小鼠胃黏膜病理组织学改变。结果①Hp感染结果:抗生素造模组小鼠胃内Hp感染率为100%,直接造模组为60%,生理盐水对照组未检测到Hp定植。抗生素造模组Hp感染率和定植量均较直接造模组明显为高(P<0.05)。②菌群分析结果:抗生素造模组小鼠胃内细菌总数为104～105CFU/g组织,直接造模组为105～106CFU/g组织,生理盐水对照组为105～106CFU/g组织。抗生素造模组的各种细菌数与直接造模组和生理盐水对照组相比显著下降(P<0.05),直接造模组和生理盐水对照组各种细菌数无显著性差异(P>0.05)。③病理组织学结果:抗生素造模组、直接造模组小鼠胃黏膜均有炎症改变,直接造模组小鼠较抗生素造模组小鼠炎症程度明显为轻(P<0.05)。生理盐水对照组未见炎症改变。结论用抗生素灌胃可破坏小鼠原籍菌群,促进Hp的定植。此种造模方法效果好于已有的Hp菌悬液直接灌胃造模法,用此法可成功建立Hp标准株感染SPF级BALB/c小鼠的造模周期短而稳定的慢性胃炎模型。Objective Achievement ratio between available method which feeding Helicobacter pylori dierectly and a new method which feeding antibiotics before Helicobacter pylori to ruin local flora in stomach were compared to establish a new Helicobacter pylori infection model which is more stable and neede less time. Methods A total of 60 SPF grade BALB/c mice were randomly divided into 3 groups. The first group of mice were pretreated with antibiotics and inoculated with H. pylori. The second group were only inoculated with H. pylori, The third group were inoculated with sodium chloride solution. Mice were sacrificed 8 weeks postinoeulation （p. i. ）. The infection of H. pylori in gastric mueosa of the mice was analyzed by rapid urease test,Giemsa staining and culture. The changes of microflora in stomach and duodenum were confirmed by selective culture. The histopathological changes in the gastric mucosa of mice were assessed in hematoxylin - eosin stained sections. Results 1. Assessment of H. pylori infection in mice. H. pylori colonization rate was 100% in the stomach of the first and the second was 60%. The quantitatation of colonized bacterial number and achievement ratio of the first group were higher than the second group. （ P 〈 0.05 ）. No H. pylori were found in the third group. 2. Assessment of microflora in stomach.. The bacterial numbers in stomach of the first group of mice was 104 - 105 CFU/g tissue and the second and the third was 105 - 106. The bacterial numbers in stomach of the first group were significantly decreased eomparise to the second and the third group（ P 〈0.05 ）. There was no significant difference between the second and the third group. 3. Assessment of Histological examination. The chronic inflammatory response in stomach of the first group was more serious than the second group （ P 〈 0.05 ）. Conclusion The gastritis model in SPF grade BALB/c mice challenged with H. pylori SS1 was successfully established in our study. With this method a new Helicobacter pylori infection m

关 键 词：幽门螺杆菌 动物模型 慢性胃炎 

分 类 号：R-332[医药卫生]