应用蛋白质组学技术分析肝癌细胞蛋白质的表达  

A Comparative Proteome Analysis of Hepatoma Cell Cytosol

作　　者：李兴[1] 潘卫[1] 邱峰[2] 邱宗荫[2] 

机构地区：[1]贵阳医学院医学检验系,贵州贵阳550004 [2]重庆医科大学药学系,重庆400000

出　　处：《贵阳医学院学报》2007年第3期221-224,共4页Journal of Guiyang Medical College

基　　金：国家自然科学基金资助项目(30476021);教育部博士点基金资助项目(教特发中心函[2004]166号)

摘　　要：目的:细胞质在细胞内的代谢过程、信号转导等功能中起着重要的作用,以体外培养的人体肝癌细胞和正常肝细胞为研究对象,运用蛋白质组学的研究方法,对比分析肝癌细胞与正常肝细胞细胞质蛋白质的差异表达情况,为肿瘤发生机制的阐释提供信息。方法:体外培养的细胞(包括肝癌细胞、正常肝细胞)以超离心分离细胞质,双向电泳分离细胞质蛋白质,图像分析肝癌细胞与正常肝细胞的差异表达蛋白质,MALDI-TOF-MS鉴定差异蛋白质。结果:在肝癌细胞与正常肝细胞的对比分析中筛选出10个差异表达蛋白质,这些差异蛋白质涉及到细胞的能量代谢、蛋白质合成、调亡调控等方面。结论:细胞癌变后细胞质中蛋白质的表达发生了许多改变,可为肿瘤发生机制的阐释提供有价值的信息。Objective： To compare the difference of cytosol protein expression between hepatoma cells and normal hepatocytes, and so as to provide contribution to the explanation of tumorigenesis and action mechanism of epirubicin. Methods： Cultured ceils （ including hepatoma cells and normal hepatocyte ） were subjected to centrifugation for the fractionation of cytosol, and the cytosol proteins were separated by using two dimensional gel electrophoresis. The expression-ahered protein spots were detected with imaging analysis, and the proteins were identified with MALDI-TOF-MS. Results： 10 expression-altered proteins were identified between the hepatoma ceils and normal hepatocytes, and were shown to be involved in energy generation, protein biosynthesis, and apoptosis regulation of the ceils. Conclusions： There occur many changes of cytosol protein expression in hepatoma cells after tumorigenesis, and the results of this study are helpful for us to understand tumorigenesis to some extent.

关 键 词：肝肿瘤 肿瘤细胞 培养的 蛋白质组 细胞质 

分 类 号：R735.7[医药卫生—肿瘤] R73-351[医药卫生—临床医学]