慢性B淋巴细胞白血病病人骨髓及外周血ZAP-70与CD38表达及意义  

EXPRESSIONS OF ZAP-70 AND CD38 IN CHRONIC B LYMPHOCYTIC LEUKAEMIA AND THEIR CLINICAL SIGNIFICANCE

作　　者：于军红[1] 孟繁军[1] 郭小芳[2] 

机构地区：[1]青岛大学医学院附属医院血液内科,山东青岛266003 [2]青岛大学医学院血液病学教研室

出　　处：《齐鲁医学杂志》2007年第4期306-308,共3页Medical Journal of Qilu

摘　　要：目的探讨ZETA相关蛋白(ZAP-70)、CD38在慢性B淋巴细胞白血病(B-CLL)病人骨髓及外周血中的表达及两者相关性,并分析其临床意义。方法采用半定量RT-PCR的方法对19例B-CLL病人及12例正常人骨髓或外周血ZAP-70 mRNA的表达水平进行测定,采用流式细胞术检测CD38的表达水平。结果在19例B-CLL病人中,7例ZAP-70阳性表达,8例CD38阳性表达;对照组ZAP-70、CD38均为阴性表达。结论ZAP-70 mRNA、CD38在B-CLL中表达阳性提示预后不良。ZAP-70可以取代IgVH基因突变状态作为B-CLL有效的预后指标。Objective To study the expressions of ZAP-70 and CD38 in chronic B lymphocytic leukaemia （B-CLL） and the correlation between them. Methods Semi-quantitative RT-PCR was utilized to analyze ZAP-70 expressions in patients with B-CLL （n=19） and normal adults （n=12）, and CD38 expression was assessed by flow cytometry. Results Positive expression of ZAP-70 was found only in seven patients with B-CLL （7/19） and CD38 in eight patients. Conclusion ZAP-70 and CD38 expressions predict poor prognosis of chronic lymphocytic leukaemia. ZAP-70 may replace IgVH mutational status to be an valid prognostic index of this disease.

关 键 词：基因 ZAP-70 基因 CD38 白血病 淋巴细胞 慢性 基因表达 预后 

分 类 号：R733.71[医药卫生—肿瘤]