胃癌组织中VEGF-C及其受体FLT-4mRNA的表达  

EXPRESSION OF VEGF-C AND ITS RECEPTOR FLT-4 mRNA IN THE GASTRIC CARCINOMA

作　　者：穆林松[1] 宋修岐[1] 孙少杰[1] 

机构地区：[1]青岛大学医学院第二附属医院普外科,山东青岛266042

出　　处：《齐鲁医学杂志》2007年第4期323-325,共3页Medical Journal of Qilu

摘　　要：目的研究血管内皮生长因子C(VEGF-C)及其受体VEGFR-3(FLT-4) mRNA在胃癌组织中的表达。方法采用逆转录聚合酶链反应技术对34例胃癌和癌旁组织及16例正常胃黏膜组织中VEGF-C及FLT-4 mRNA的表达水平进行检测。结果VEGF-C与FLT-4 mRNA在胃癌和癌旁组织中均有表达;VEGF-C及FLT-4 mRNA在胃癌、癌旁组织和正常胃黏膜组织中的表达有明显差异(χ2=23.87、18.97,P<0.05);VEGF-C及FLT-4 mRNA在胃癌组织中的表达呈明显正相关(r=0.67,P<0.05);VEGF-C及FLT-4 mRNA的表达与胃癌淋巴转移均呈明显正相关(r=0.52、0.52,P<0.05);VEGF-C mRNA在高、中和低分化胃癌组织中的表达有明显差异(F=21.04,P<0.05);BorrmannⅠ、Ⅱ、Ⅲ、Ⅳ型胃癌组织中VEGF-C mRNA的表达有明显差异(F=57.94,P<0.05)。结论VEGF-C及其受体FLT-4在胃癌的发生发展过程中可能起一定的作用;两者高表达可能与胃癌淋巴结的转移有关。Objective To investigate the expression of vascular endothelial growth factor-C （VEGF-C） and its receptor VEGFR-3 （FLT-4） at the mRNA level in gastric carcinoma. Methods Expressions of VEGF-C and FLT-4 were studied by semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR） among patients with gastric carcinoma （n = 34） and adjacent normal tissues from the same patients. Results VEGF-C and FLT-4 were expressed in the tissue of the cancer and adjacent tissue. Significant differences in expressions of VEGF-C and FLT-4 were noticed among the cancer, adjacent tissue and normal tissue samples （χ^2 = 23.87,18.97;P〈0.05）, and a positive correlation between VEGF-C and FLT-4 expression observed in the tissue of the cancer （r= 0, 67, P〈0.05）. With regard to VEGF-C and FLT-4 expression, there was a significant difference between positive and negative lymph node metastases （r=0.52;0.52,P〈0.05）. Conclusion VEGF-C and its receptor FLT-4 may play an important role in the development of gastric cancer, and the over expression of both of them are more likely involved in lymph node metastasis.

关 键 词：胃肿瘤 血管内皮生长因子C 基因表达 

分 类 号：R735.1[医药卫生—肿瘤]