冬凌草甲素对肝癌HepG2细胞株体外放射增敏作用  

RADIOSENSITIZATION EFFECT OF ORIDONIN ON HepG2 IN VITRO

作　　者：王昊[1] 于洪升[1] 薛宏伟[1] 

机构地区：[1]青岛大学医学院附属医院肿瘤科,山东青岛266003

出　　处：《齐鲁医学杂志》2007年第4期339-340,342,共3页Medical Journal of Qilu

摘　　要：目的观察冬凌草甲素对肝癌细胞株体外的放射增敏作用。方法对肝癌细胞系(HepG2)进行克隆形成实验。照射前用1.804、3.608、5.412、7.216、18.040μmol/L冬凌草甲素处理HepG2细胞6～72h,然后分别给予0、1、2、3、4、5、6Gy的^(60)Co照射,观察细胞存活率,计算放射增敏比(SER)。并运用常规照射剂量2Gy时的放射增敏比(SERSF2)作为评价指标。结果冬凌草甲素可提高HepG2细胞的放射敏感性,并呈时间、浓度依赖关系。照射前用1.804μmol/L冬凌草甲素处理HepG2细胞6～72h,在6、12和24h未观察到放射增敏作用,放射增敏作用仅在48和72h出现。当高浓度冬凌草甲素(18.040μmol/L)处理HepG2细胞6、12和24h,各时间点均可见放射增敏效应。结论冬凌草甲素可能是一种肝癌放射增敏剂。Objective To investigate the radiosensitization effect of Oridonin on hepatic cancer cell lines （HepG2） in vitro. Methods Clonogenic assay was used to get HepG2 cell line. Different doses of oridonin （1. 804,3. 608,5. 412,7. 216 and 18. 040 μmol/ L） were used to treat HepG2 cells for 6-72 h, before radiation of ^60Co in doses of 0, 1, 2, 3, 4, 5, 6 Gy, respectively. The survival fraction was observed, the sensitization enhancement ratio （SER） calculated, and changes in radiosensitivity was estimated by SER at a clinically relevant dose of 2 Gy （SERSF2）. Results Oridonin enhanced the radiosensitivity of HepG2 cells in a time-and dose-dependent manner. Enhancement of radiosensitivity was observed at 48 h and 72 h after treated with low dose of oridonin （1. 804 μmol/L）. High dose of oridonin （18. 040 μmol/L） produced early radiosensitivity effect observed at 6, 12 and 24 h. Conclusion Oridonin might be a potential radiosensitizer in hepatic cancer cell lines.

关 键 词：冬凌草甲素 辐射增敏药 细胞系 肿瘤 辐射增敏 

分 类 号：R730.55[医药卫生—肿瘤]