ε-聚赖氨酸测定方法的改进  

Improvement of the Assay Method forε-polylysine

作　　者：曹伟锋[1] 谭之磊[1] 袁国栋[1] 贾士儒[1] 

机构地区：[1]天津科技大学生物工程学院天津市工业微生物重点实验室,天津300457

出　　处：《天津科技大学学报》2007年第2期9-11,32,共4页Journal of Tianjin University of Science & Technology

基　　金：国家"十五"科技攻关项目(2004BA713B05-061);天津科技大学自然科学基金资助项目(20060207)

摘　　要：研究了甲基橙法测定ε-聚赖氨酸的试验条件,得出结论:甲基橙从水中重结晶后,溶于pH6.6、0.1 mol/L磷酸钠缓冲液中,终浓度为1 mmol/L;将2 mLε-聚赖氨酸溶液与2 mL 1 mmol/L甲基橙溶液混合;混合物在30℃条件下剧烈振荡30 min,4 000 r/min离心15 min;取1 mL上清液稀释50倍,在465 nm波长下测吸光度,计算ε-聚赖氨酸含量.The conditions of detecting ε-polylysine （ε-PL） concentration by the methyl orange were studied in the paper. The results were as follow. After recrystallization from water, methyl orange was dissolved in 0.1mol/L sodium phosphate buffer （pH6.6） at a final concentration of 1 mmol/L. Then 2ml ε-PL solution was mixed with 2ml methyl orange solution. The mixtures were vigorously reacted on a reciprocal shaker at 30℃ for 30min and then centrifuged for 15min at 4000 r/min. The absorbance of the supernate diluted 50-fold was read at 465nm and the ε-PL content was calculated.

关 键 词：甲基橙 Ε-聚赖氨酸 比色法 

分 类 号：O657.3[理学—分析化学]