毛细管电泳-激光诱导荧光检测大鼠脑组织多巴胺含量  

Determination of dopamine in the brain tissue of rats by using capillary electrophoresis with laser-induced fluorescence method

作　　者：刘广印[1] 沈佐君[1] 何晓东[1] 伊茂礼[1] 

机构地区：[1]安徽省立医院临床检验中心,安徽合肥230001

出　　处：《蚌埠医学院学报》2007年第4期393-395,共3页Journal of Bengbu Medical College

基　　金：安徽省自然科学基金资助项目(050430902)

摘　　要：目的:采用毛细管电泳-激光诱导荧光法建立检测大鼠脑组织多巴胺含量的方法。方法:低温匀浆大鼠脑组织离心后,取上清用pH11.010mmol/L硼砂缓冲液,在室温下衍生反应10h连接上FITC荧光基团;毛细管电泳分离条件为pH10.3100mmol/L硼酸-Tris缓冲液。结果:在选定的实验条件下,方法检测限(S/N=3)达2.1nmol/L水平,批内相对标准差(RSD)为3.4%,回收率达96.05%,在10-8～10-10mol/L范围内呈良好线性关系。结论:应用毛细管电泳-激光诱导荧光法可检测鼠脑组织多巴胺含量。Objective：To establish a method of determining the dopamine in the brain tissue of rats by using capillary electrophoresis with laser-induced fluorescence. Methods：The homogenate of the rats＇ brain tissue was centrifugated in low temperature and the supernatant was reacted in pH 11.0 10 mmol/L borax buffer solution. The reaction of derivatization time was 10 hours under alkaline （10 mmol/L Na2 B407 pH 11.0） ; mobile condition of capillary electrophoretic separation was a mixture of 100 mmol/L Boric acid- Tris. Results：Detection limits of the method were determined to be lower than 2. 1 nmol/L and the RSD values were 3.4% ; the recoveries were above 95.6%. The linearity was from 10 ^-10 to 10^-8 mol/L. Conclusions：The method of detecting dopamine in the brain of rats is convenient by using capillary electrophoresis with laser-induced fluorescence.

关 键 词：帕金森病 多巴胺 电泳 毛细管 激光诱导荧光 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R971.93[医药卫生—临床医学]