柑橘及其近缘属植物LFY同源基因的比较  

Comparative study of LFY homologous genes among Citrus and its relative genre plants

作　　者：何新华[1,2] 郭永泽[3] 佘金彩[3] 李杨瑞[1] 

机构地区：[1]广西农业科学院作物遗传改良与生物技术重点实验室 [2]广西大学农学院,南宁530004 [3]广西大学农学院

出　　处：《果树学报》2007年第4期521-524,共4页Journal of Fruit Science

基　　金：广西自然科学基金(桂科字0542022);广西科学基金应用基础研究(桂科基073104);广西农科院博士后基金

摘　　要：LFY基因处于成花调控网络的关键位置,不仅调控开花时间和花转变,而且在花序和花的发育中也起重要作用。为了进一步探讨柑橘及其近缘属植物开花的分子机理,利用PCR技术分别从兴津温州蜜柑(Citrus unshiuMarcovitch)、无核椪柑(Citrus reticulata Blanco)、沙田柚[Citrus grandis(L.)Osbeck]、融安金柑(Fortunella crassifoliaSwing)和无核黄皮[Clausena lansium(Lour.)Skeels]叶片中分离克隆了LFY全长同源基因。结果表明兴津温州蜜柑、无核椪柑、沙田柚、融安金柑和无核黄皮中的LFY全长同源基因的核苷酸长度分别为2090、2086、2092、2081、2089bp,分别编码398、398、398、398和397个氨基酸,这些同源基因均由3个外显子和2个内含子组成。同源性分析发现,这些LFY全长同源基因的核苷酸序列和氨基酸序列同源性高,分别为92%～99%和95%～100%。亲缘关系分析结果与当前的植物学分类结果一致。LFY gene not only controls flowering time and floral transition, but also plays an important role in inflorescence and floral organ development. It is situated at the central site in gene network of flowering regulation. In order to probe further the molecular mechanism among Citrus and its relative plants, complete LFY homologous genes from Satsuma （C. unshiu Marcovitch）,Ponkan（C. reticulata Blanco）, Shatian pomelo [C. grandis （L.） Osbeck], Kumquat （Fortunella crassifolia Swingle） and Wompee [Clausena lansiurn （Lour.） Skeels] were isolated and cloned by polymerase chain reaction （PCR）. The results showed that the nucleotide size of complete LFY homologous genes from Satsuma, Ponkan, Shatian pomelo, Kumquat and Wompee was 2 090, 2 086, 2 092, 2 081 and 2 089 bp respectively and they encoded 398,398,398,398 and 397 amino acids respectively. These complete LFY homologous genes were all composed of 3 exons and 2 introns. The homologies of nucleotide and amino acid sequences among the complete LFY homologous genes were 92% to 99% and 95% to 100% respectively. The result of phylogenetic tree based on amino acid sequence homology among LFY homologous genes is similar to the result of plant taxonomy.

关 键 词：柑橘 金柑 黄皮 LFY 

分 类 号：S666[农业科学—果树学]