应用基因芯片检测动物性食品中主要致病菌  

Main pathogenic bacteria of materials in the animal origin food detected by gene chip

作　　者：王金玲[1] 曹际娟[2] 乔洪伟[1] 韩俊友[1] 王芳[3] 贾雁[1] 于凤琴[3] 韩文瑜[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]辽宁出入境检验检疫局,辽宁大连116015 [3]沈阳出入境检验检疫局,辽宁沈阳110016

出　　处：《中国兽医学报》2007年第4期485-489,共5页Chinese Journal of Veterinary Science

基　　金：中央级科研院所社会公益研究专项国家科技基础条件平台工作重点项目(2003DIA6N002-002-05)

摘　　要：依据16SrDNA、23SrDNA上的恒定区和可变区设计7种致病菌的通用引物和特异性寡核苷酸探针,并对探针5’端进行氨基化修饰进行基因芯片共价结合,然后优化杂交反应液、芯片点样及后处理程序,研制出一种可同时检测动物性食品中7种主要致病菌的基因芯片。结果表明,7种致病菌的基因芯片杂交检测灵敏度可达102cfu/g,与经典传统检测方法及分子生物学、免疫学方法相比该方法特异性强,准确率高,可同时检测动物性食品中7种致病菌。The common primer and specific oligonucletide probes were designed and synthesized for seven pathogenic bacteria according to the constant regions and variable regions of 16s ribosomal DNA and 23s ribosomal DNA,and amination modification of probe 5＇end to covalent bond to the gene chip,then select hybridization reaction liquid and array apotting and postprocessing, a set of gene chip were developed for detection of seven main pathogenic bacteria in animal origin food . The result showed that the sensitivity of this identification system reached 10^2 cfu/g. Compared to the classical traditional,molecular biology and immunology method,its specificity and accuracy is higher for detection of the seven pathogenic bacteria in the animal origin food.

关 键 词：基因芯片 动物性食品 主要致病菌 

分 类 号：Q78[生物学—分子生物学]