富硒麦芽对二乙基亚硝胺致SD大鼠肝癌细胞因子和血管生成的影响  

Effect of selenium-enriched malt on the cytokines and angiogenesis of diethylnitrosamine-induced hepatocarcinoma rats

作　　者：刘家国[1] 赵洪进[1] 刘艳娟[1] 王小龙[1] 

机构地区：[1]南京农业大学畜禽营养代谢病研究室,江苏南京210095

出　　处：《中国兽医学报》2007年第4期573-578,共6页Chinese Journal of Veterinary Science

基　　金：江苏省"九五"农业重点攻关资助项目(Q9837);高校博士点基金资助项目(20020307022);南京农业大学青年科技创新基金资助项目(KJ05015)

摘　　要：雄性SD大鼠193只，体质量100～120g，随机分为5组。Ⅰ～Ⅲ组，日粮中添加富硒麦芽至硒含量分别为0．3、1．0、3．0mg／kg；Ⅳ组为模型组；Ⅴ组为正常对照组，不加硒也不诱癌，日粮中分别添加亚硒酸钠至硒含量达0．1mg／kg。Ⅰ～Ⅳ组，饮水中添加二乙基亚硝胺（diethylnitrosamine，DEN，100mg／L）诱癌，维持DEN摄入量按体质量每天约10mg／kg，连续16周，然后改饮普通灭菌水至18周末。Ⅴ组始终以普通灭菌水自由饮用。每4周每组取5只鼠眼眶采血；18周末采血、处死所有大鼠。测定血清肿瘤坏死因子α（tumor necrosis factor-α，TNFα）、白介素-2（interleukin-2，IL-2）、胰岛素样生长因子-Ⅱ（insulin—like growth factor-Ⅱ，IGF-Ⅱ）、一氧化氮（nitricoxide，NO）、一氧化氮合成酶（nitric oxide synthase，NOS）等的含量，并以免疫组化法观察大鼠肝肿瘤组织血管内皮生长因子（vascular endothelial growth factor，VEGF）的表达。结果发现，某些剂量富硒麦芽能有效地抑制肝癌大鼠TNFα、IGF-Ⅱ、NO和NOS水平的上升，提升血清IL-2水平，抑制肝肿瘤组织中VEGF的表达。结果提示，富硒麦芽能促进肝癌大鼠免疫功能，抑制肿瘤血管生成，从而发挥其抗癌作用。One hundred and ninety-three SD rats weighting 100-120 g were divided randomly into 5 groups. The animals in group Ⅰ , Ⅱand Ⅲ were fed with selenium as Se-enriched malt （SEM）supplemented diets （0.3, 1.0 and 3.0 mg/kg）. Animals in group Ⅳ and group Ⅴ received 0.1 mg/kg selenium as sodium selenite only and were served as the positive control and negative control respectively. Hepatocarcinoma was induced in animals of groups Ⅰ - Ⅳ by diethylnitrosamine diluted in the sterilized water （100 mg/L） at the dose of 10 mg/kg body weight every day for 16 weeks, then drunk the sterilized water for another 2 more weeks. Subsequently, five animals in each group were sacrificed by cervical decapitation in every 4 weeks, and diet was deprived in all the rats for 12 hours and sacrificed by cervical decapitation at the end of the 18th week. Then the effects of SEM on the items, including TNFα, IL-2, IGF- Ⅱ , NO and NOS levels in plasma were recorded. At the same time, the protein levels of vascular endothelial growth factor （VEGF） in tumor tissue were analyzed by immunohistochemistry. The results indicated that SEM was effective in decreasing the levels of TNFa.IGF- Ⅱ , NO and NOS, and increasing the contents of IL-2 in the plasma, inhibiting the expression of VEGF in tumor tissue. In conclusion, SEM could delay efficiently the development of hepatocarcinoma in rats by promoting the immune function and inhibiting the angiogenesis.

关 键 词：SD大鼠 富硒麦芽 白介素-2 胰岛素样生长因子-Ⅱ 血清肿瘤坏死因子Α 一氧化氮 一氧化氮合成酶 血管内皮生长因子 

分 类 号：S816.72[农业科学—饲料科学] S852.16[农业科学—畜牧学]