Protection of preconditioning,postconditioning and combined therapy against hepatic ishemia/reperfusion injury  

作　　者：武步强 褚薇薇 张六一 王鹏 马秋野 王殿华 

机构地区：[1]Department of General Surgery Affiliated Heping Hospital,Changzhi Medical College [2]Key Pharmacological Laboratory of Natural Products Kunming Medical College

出　　处：《Chinese Journal of Traumatology》2007年第4期223-227,共5页中华创伤杂志（英文版）

摘　　要：Objective： To study the protective effect of ischenlic preconditioning （I-pre） and ischemic postconditioning （I- post） against ischenlia/reperfusion （I/R） injury in rat＇ s liver. Methods： Using rat model of hepatic segmental I/R injury, rats were divided into 5 groups： Group A （sham group）, Group B （I/R injury）, Group C （I-pre group）, Group D （I-post group ） and Group E （combined treatment of I-pre and I-post ）. Serum alanine aminotransferase （ ALT ）, aspartate aminotransferase （ AST）, malondiaidehyde （ MDA ）, glutathione （ GSH ）, superoxide dismutase （ SOD ）, glutathione peroxidase （GSH-Px） and myeloperoxidase （MPO） in hepatic tissues were determined, respectively. In addition, 7 days＇survival of Groups B, C, D and E were evaluated. Results. Compared with Group B, Groups C, D and E exhibited significantly decreased ALT and AST release, minimized tissue injury, suppressed values of MDA and MPO, increased activities of SOD, GSH-Px and GSH （P〈0.05 ）, as well as improved animal survival. The differences among Groups C, D and E were not statistically significant. Conclusions： I-pre, I-post and combined therapy of I-pre and I-post have protective effect against hepatic I/R injury, which is correlated with its function of reducing the production of reactive oxygen species, maintaining the activities of antioxidant systems and suppressing neutrophils recruitment. No additive effect can be obtained in Group E.

关 键 词：LIVER Reperfusion injury Ischemic preconditioning Ischemic postconditioning 

分 类 号：R575[医药卫生—消化系统]