姬松茸液体菌种制备初探  

Production of Agaricus blazei Liquid Spawn

作　　者：周全[1] 唐兴国[1] 姚志伟[1] 高军伟[1] 

机构地区：[1]武汉生物工程学院生物工程系,湖北武汉430415

出　　处：《食用菌学报》2007年第2期77-80,共4页Acta Edulis Fungi

基　　金：武汉市市属高等学校2006年度科学研究项目"巴西蘑菇标准化生产体系的建立"[武教高(2006)5号]的部分研究内容

摘　　要：通过正交试验筛选出适宜培养姬松茸液体菌种的合成培养基(N6Ⅰ20.0%,N6Ⅱ30.0%,蔗糖5.0%,酵母粉0.3%),以此制备的一级摇瓶种和二级摇瓶种,其菌丝体生物量分别为1.48±0.06g/100mL和2.64±0.05g/100mL。栽培试验表明,液体菌种的发菌时间比固体菌种缩短13d。A synthetic liquid medium consisting of 20.0% N6 I （mineral salt solution）, 30.0% N6 I[ （vitamin/ amino acid supplement）, 5.0% sucrose, 0.3% yeast powder was selected by orthogonal testing for culturing Agaricus blazei mycelium. Mycelial yields from shaken primary cultures （50 mL culture medium in 250 mL culture bottles） incubated at 25 ℃ for five days were 1. 48 -0. 06 g/100 mL. Mycelial yields obtained following transfer of mycelium from primary cultures to secondary cultures （70 mL in 250 mL culture bottles） incubated at 25 ℃ for five days were 2.64 5±0.05 g/100 mL. Colonization of cultivation substrate using the liquid spawn from secondary cultures occurred 13 d earlier compared with normal stock spawn.

关 键 词：姬松茸 N6培养基 摇瓶培养 液体菌种 

分 类 号：S646.1[农业科学—蔬菜学]