葡萄糖氧化酶在管状空心SiO_2载体上的固定化  

Hollow silica nanotubes for immobilization of glucose oxidase enzyme

作　　者：蒋利伟[1] 袁其朋[1] 肖清贵[2] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029 [2]北京化工大学教育部超重力工程研究中心,北京100029

出　　处：《北京化工大学学报（自然科学版）》2007年第4期428-431,共4页Journal of Beijing University of Chemical Technology(Natural Science Edition)

基　　金：国家自然科学基金(20376007)

摘　　要：以针状CaCO3为无机模板,制作成新型固定化材料管状空心SiO2载体,用来固定葡萄糖氧化酶(GOD)。研究了pH值,温度和载体与GOD的配比等对固定化酶活的影响。所得的最佳工艺条件为:最佳载体和游离酶配比为0.6 g/mL;固定化酶的最适pH值为5.2;最佳反应温度为32℃。和游离酶相比无论操作性还是稳定性都有提高。Porous hollow silica nanotubes （PHSNTs） have been synthesized via a sol-gel route using nano-sized needle-like CaCO3 as the inorganic template and employed as a support for immobilization of Glucose Oxidase enzyme （GOD） biocatalyst. The effect of varying factors such as phosphate buffer pH, immobilization temperature and ratio of carrier to free GOD （g/mL） on the activity of immobilized GOD are discussed. The optimum conditions were found to be as follows： ratio of carrier to free GOD （g/mL） 0.6 g/mL, phosphate buffer pH 5.2, immobilization temperature 28℃. The results show that under optimized conditions the efficacy and stability of GOD are improved relative to the free enzyme.

关 键 词：固定化酶 葡萄糖氧化酶 管状空心SiO2 针状碳酸钙 

分 类 号：Q814.2[生物学—生物工程]