复发/难治性急性淋巴细胞白血病患者多药耐药基因表达及临床意义  

Detection and clinical significance of mdr1 gene in relapse or refractory ALL patients

作　　者：朱莉 张丽[2] 李光耀[2] 王美芝[1] 马霖[1] 陈雪红[3] 张海平[1] 姚如永[1] 韩志武[1] 赵洪国[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003 [2]聊城市人民医院 [3]青岛大学医学院

出　　处：《山东医药》2007年第17期1-3,共3页Shandong Medical Journal

基　　金：国家"九五"医学科技攻关基金资助项目(96-906-01-23)

摘　　要：目的探讨复发/难治性急性淋巴细胞白血病(ALL)患者多药耐药基因(mdr1基因)的表达及其临床意义。方法采用Northern blot法检测K562/A02细胞株mdr1基因的表达,RT-PCR法检测42例ALL患者(ALL组)、27例非白血病患者和健康人(对照组)mdr1基因的表达水平,分析mdr1基因过度表达的临床意义。结果ALL组mdr1基因过度表达率显著高于对照组(P<0.01),其中复发/难治者高于初诊和完全缓解(CR)者(P<0.01),临床耐药者高于非临床耐药者(P<0.01);非临床耐药者CR率显著高于临床耐药者(P<0.001)。结论mdr1基因过度表达与ALL患者临床耐药密切相关,是影响预后的重要因素;mdr1基因表达可作为判断ALL患者临床耐药和预后的指标。[Objective] To study the expression of multidrug resistance gene （mdrl） and its clinical signihcance. [Methlods] A semi-quantitative reverse transcription polymerase chain reaction（RT-PCR）was used to investigate the transcription levels of the human sorcin gene detected by Northern blot in 42 ALL patients and 27 controls,and analyzed the clinical significance of mdrl gene. [Results] Mdrl gene expression was significantly higher in ALL patients than that in normal controls （P〈0.01）,and higher in relapsed/refractory ALL patients than that in those newly diagnosed or in complete remission. Mdrl gene expression was significantly lower in non-resistant patients than that in resistant ones （P〈0. 01）. CR rates of non-resistant patients was higher than of resistant ones （P〈0. 001）. [Conclusion] Mdrl gene overexpression is significantly associated with clinical multidrug resistance and prognosis, and it is one of the indicators for multidrug resistance and prognosis of ALL patients.

关 键 词：白血病 淋巴细胞性 复发/难治 多药耐药基因 

分 类 号：R733.3[医药卫生—肿瘤]