吗啡及一氧化氮合酶抑制剂对小鼠胸腺细胞诱导型一氧化氮合酶mRNA表达的影响  

Effect of morphine and L-NAME on the expression of iNOS mRNA in thymocyte in mice

作　　者：姜关俊[1] 苏中华[2] 刘学兵[3] 郝伟[4] 

机构地区：[1]广东省人民医院、广东省精神卫生研究所,广州510120 [2]山东省济宁市精神病防治院 [3]武汉精神卫生中心 [4]中南大学湘雅二医院精神卫生研究所

出　　处：《中国行为医学科学》2007年第7期583-585,共3页Chinese Journal of Behavioral Medical Science

基　　金：国家重点基础研究项目（2003CB515400）;国家自然科学基金（30370522）

摘　　要：目的探讨一氧化氮合酶抑制剂L—NAME对吗啡依赖小鼠胸腺细胞中诱导型一氧化氮合酶（iNOS）mRNA表达的影响。方法雌性Balb／C小鼠40只，随机分为吗啡组、吗啡＋纳络酮组、吗啡＋L—NAME组和对照组。吗啡腹腔注射建立吗啡依赖小鼠模型，在干预组分别给予纳络酮和L-NAME，分别于首次用药后24h和终止处理因素后6h，随机各组均取半数颈椎脱臼处死取出胸腺，采用原位杂交方法和图像分析技术测定各标本杂交阳性细胞的光密度值，进行统计分析。结果1．与对照组（OD=0．1932±0．0086）比较，首次用药后24h和终止处理因素后6h时，吗啡组和吗啡＋L-NAME组胸腺切片杂交信号强度均显著升高（OD=0．2245±0．0098，F=37．894，P〈0．01；OD=0．2207±0．0091，F=36．023，P〈0．01），而吗啡＋纳洛酮组胸腺切片杂交信号无显著差异；2．与吗啡组比较，首次用药24h后和终止处理因素后6h时，吗啡＋纳洛酮组杂交信号均显著降低（OD=0．1969±0．0087，F=4．659，P〈0．05；OD=0．2024±0．0085，F=46．816，P〈0．01），吗啡＋L-NAME组杂交信号均无显著差异（OD=0．2443±0．0087，F=3．278，P〉0．05）。结论高剂量吗啡能诱导小鼠胸腺细胞iNOSmRNA表达，而阿片受体拮抗剂纳洛酮能较好地阻断该效应，但NOS抑制剂L-NAME则对小鼠胸腺细胞iNOSmRNA表达无明显影响。Objective To study the expression of the iNOS mRNA on different timepoints in thymoeyte in morphine-dependent mice. Methods Forty female Balb/C mice were randomly divided into four groups as morphine, morphine plus naloxone, morphine plus L-NAME and control groups. Morphine was injected i.p. to develop morphine-dependent model. Naloxone and L-NAME were injected i.p. respectively as intervention. Half number mice were randomly sacrificed by cervical vertebra dislocation on the point of treated for 24h and 6h after the last injection. Thymus were immediately taken out under sterile condition. Thymus slides were detected by in situ hybridization and quantitative analysis which indicated the level of mRNA. And data were analyzed on computer with SPSS11.5. Results 1. After treated for 24h, both morphine group and morphine plus L-NAME groups showed a significantly higher hybridization signal than control （ OD = 0. 2245 ± 0. 0098, F= 37. 894, P〈 0.01 ; OD = 0. 2207 ± 0. 0091, F = 36. 023, P 〈 0.01 ）, while no significant difference was detected between morphine plus naloxone group and control. Morphine plus naloxone group showed a significantly lower signal than morphine group, while no difference detected between morphine plus L-NAME group and morphine group. 2. Six hours after the last injection, there showed a significantly higher level in both morphine group and morphine plus L-NAME group than control（ OD = 0. 1969 ± 0. 0087, F = 4. 659, P 〈 0.05 ; OD = 0. 2024 ± 0. 0085, F = 46. 816, P 〈 0.01 ） , while no difference was found between morphine plus naloxone group and control. The signal level was significantly lower in morphine plus naloxone group than morphine group, while no difference was found between morphine plus L-NAME group and morphine group. Conclusion Expression of iNOS in thymocyte can be increased by morphine, which can be blocked by μ-receptor antagonist naloxone, not NOS inhibitor L-NAME.

关 键 词：吗啡依赖 纳洛酮 诱导型一氧化氮合酶 L-NAME 原位杂交 

分 类 号：R743.31[医药卫生—神经病学与精神病学]