神经肽Y诱导大鼠心肌细胞内游离钙重分布研究  

Neuropeptide Y induced redistribution of intracellular free calcium in rat cardiomyocytes

作　　者：熊龙根[1] 张弋[1] 苏涛[2] 刘世明[1] 董颀[3] 陈敏生[1] 

机构地区：[1]广州医学院第二附属医院心内科,广东广州510260 [2]广州医学院第二附属医院神经科学研究所,广东广州510260 [3]广州医学院生理教研室,广东广州510182

出　　处：《中国病理生理杂志》2007年第7期1254-1257,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No30440053;No30570759);广东省自然科学基金资助项目(No04004529)

摘　　要：目的:观察神经肽Y(NPY)对大鼠心肌细胞胞浆钙浓度和肌浆网(SR)内钙含量的影响。方法:用100nmol.L-1NPY刺激Sprague-Dawley乳鼠心肌细胞24h,用荧光染料Fluo-4AM负载胞浆钙,记录静息状态下心肌细胞胞浆钙浓度,并用咖啡因诱导的胞浆钙瞬变幅度来反映肌浆网内总钙负荷;用荧光染料Fluo-5NAM直接标记心肌细胞肌浆网内游离钙离子。所有钙影像均由LeicaSP2激光共聚焦显微镜记录。结果:100nmol.L-1NPY刺激24h后,心肌细胞胞浆游离钙浓度明显高于对照组(P<0.05);心肌细胞肌浆网内游离钙含量明显低于对照组(P<0.01);咖啡因诱导下钙瞬变幅度也低于对照组。结论:24hNPY刺激可导致心肌细胞内游离钙出现空间分布的变化,即胞浆钙浓度增高而肌浆网内钙负荷减少。AIM： To investigate the effect of neuropeptide Y （NPY） on intracellular free calcium （ [ Ca^2＋]i ） and Ca^2＋i sarcoplasmic reticulum of cardiomyocytes in rats. METHODS ： Cardiomyocytes of neonatal Sprague - Dawley rats were incubated with NPY at concentration of 100 nmol/L for 24 h. Fluorescent indicator Fluo -4 AM was used to detect [ Ca^2＋]i and Fluo -5N AM was used to detect Ca^2＋ in sarcoplasmic reticulum （SR）. Calcium image was recorded by laser scanning confocal microscope. The SR Ca^2＋ load was estimated by caffeine -induced Ca^2＋ transient （CCT）. RESULTS： 24 h after incubation with NPY, compared with control group, the concentration of [ Ca^2＋] i was significantly elevated （ P 〈 0. 05 ）, and the concentration of free Ca^2＋ in SR （ [ Ca^2＋ ] sa ） was significantly decreased （ P 〈 0. 05 ）, and the peak of CCT was attenuated. CONCLUSION ： Stimulation with NPY for 24 h causes redistribution of free calcium in rat cardiomyocytes, namely the elevation in [ Ca^2＋]i and decline in [ Ca^2＋] sa.

关 键 词：神经肽Y 心肌细胞 肌浆网 钙 

分 类 号：R363[医药卫生—病理学]