三种中药复方对慢性束缚应激大鼠皮层和海马BDNF、TrkB的影响  

Effects of three Chinese formulas on BDNF,TrkB in rat contex and hippocampus with chronic immobilization stress

作　　者：陈家旭[1] 李伟[1] 赵歆[1] 徐洪雁[1] 王竹风[1] 岳广欣[1] 杨建新[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中国病理生理杂志》2007年第7期1296-1300,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No30000216;30672578);高等学校全国优秀博士学位论文作者专项资金资助项目(No200059);霍英东教育基金会资助项目(No81037);高校青年教师奖资助项目

摘　　要：目的:研究慢性束缚应激时大鼠皮层和海马BDNF、TrkB的变化以及逍遥散、四君子汤、金匮肾气丸3种中药复方对其影响。方法:用特制束缚架连续束缚7d与21d,每天3h的方法制作大鼠束缚应激模型,用免疫组织化学方法结合图像分析检测大鼠皮层和海马CA1区BDNF、TrkB的变化。结果:连续束缚7d、21d后大鼠大脑额叶皮层与海马CA1区的BDNF均显著低于正常对照组(P<0.05,P<0.01),尤以21d模型组明显。连续束缚7d、21d后大鼠大脑额叶皮层与海马CA1区的TrkB分别显著高于正常对照组(P<0.05,P<0.01)。3个中药复方均能升高皮层BDNF的积分吸光度和海马中BDNF的阳性细胞数;逍遥散能降低海马和皮层中的TrkB阳性细胞数和海马TrkB的积分吸光度;四君子汤和金匮肾气丸能降低皮层TrkB的积分吸光度;金匮肾气丸能降低皮层TrkB的阳性细胞数;逍遥散升高皮层和海马中BDNF的作用比四君子汤和金匮肾气丸明显。结论:皮层和海马CA1区BDNF下降参与慢性应激的变化,疏肝、健脾、补肾的中药复方均有一定程度的逆转作用,但以逍遥散的作用较强,优于四君子汤和金匮肾气丸。AIM： To investigate the changes of brain - derived neruotrophic factor （BDNF） , tyrosine kinase B （TrkB） in rat cortex and hippocampus with chronic immobilization stress and the influence of three Chinese formulas （ Xiaoyaosan, Sijunzitang, Jinkuishenqiwan） on them. METHODS ： Chronic immobilization stress method （ 180 min daily, repeated 7 days or 21 days） was taken, and the changes of BDNF, TrkB in rat forehead cortex and hippocampus CA1 were measured by immunohistochemistry integrated image analysis. RESULTS： The contents of BDNF in rat forehead cortex and hippocampus CA1 were obviously lower in the model group of 7 days and 21 days than those in the normal control group （P 〈0. 05, P 〈0. 01 ）, especially the lowest in the model group of 21 days. The contents of TrkB in rat forehead cortex and hippocampus CA1 were higher in the model group of 7 days and 21 days than those in the normal control group （ P 〈0. 05, P 〈0. 01 ）. All three Chinese formulas increased the intergral absorbance of BDNF in rat cortex and particle number of BDNF in rat hippocampus CA1. The particle number of TrkB in rat hippocampus CA1 and cortex, and intergral absorbance of TrkB in rat hippocampus CA1 were reduced by Xiaoyaosan. The intergral absorbance of TrkB in rat forehead cortex was reduced by Sijunzitang and Jinkuishenqiwan. The particle number of TrkB in rat forehead cortex was decreased by Jinkuishenqiwan. Compared with the influence of BDNF in response to three Chinese formulas, effect of Xiaoyaosan was more remarkable. CONCLUSION： Decreased BDNF in rat forehead cortex and hippocampus CA1 may participate in changes of chronic immobilization stress, and it can be reversed by the Chinese herbs of soothing liver, strengthening spleen, nourishing kidney, but the effect of Xiaoyaosan is better than that of Sijunzitang and Jinkuishenqiwan.

关 键 词：应激 脑源性神经营养因子 蛋白质酪氨酸激酶 中草药 

分 类 号：R363[医药卫生—病理学]